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Fluorescence resonance energy transfer using DNA-templated copper nanoparticles for ratiometric detection of microRNAs
Analyst ( IF 3.6 ) Pub Date : 2021-2-10 , DOI: 10.1039/d0an02371j
Seokjoon Kim 1, 2, 3, 4, 5 , Ki Soo Park 1, 2, 3, 4, 5
Affiliation  

We examined the effectiveness of a ratiometric method using DNA-templated copper nanoparticles, which can function as a probe for fluorescence resonance energy transfer. This method in combination with PCR successfully detected the target microRNA, which corresponded well with the results obtained by quantitative reverse transcription PCR.

中文翻译:

使用以DNA为模板的铜纳米颗粒进行荧光共振能量转移,用于比例检测microRNA

我们检查了使用DNA模板铜纳米颗粒的比例法的有效性,该铜纳米颗粒可以用作荧光共振能量转移的探针。此方法与PCR结合成功地检测了目标microRNA,与通过定量逆转录PCR获得的结果非常吻合。
更新日期:2021-02-19
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