Abstract

A rapid and highly sensitive modified QuEChERS method was combined with high performance liquid chromatography-ultraviolet fluorescence detection (HPLC-UV-FLD) to determine and evaluate sixteen polycyclic aromatic hydrocarbons (PAHs) in 74 traditional Chinese medicines (TCMs). A sample-based QuEChERS method pretreatment technology was used to improve sensitivity and selectivity through decolorization and purification in five different medicinal parts of plant TCMs. The limits of detection (LODs) ranged from 0.03 to 0.60 μg/kg in dry TCMs. All linearity coefficients (r²) for PAHs exceeded 0.999 within the linear range from 0.1 to 1000 ng/mL. The spiked recoveries in five matrices were within the range 84.1–98.8%. The relative standard deviations (RSDs) ranged from 1.2% to 8.3%. Spiked recovery tests were done in five parts of dry matrices to conclusively validate the accuracy of the method. The total PAHs ranged from 1.4 to 156.0 μg/kg. Among the 16 PAHs, the detection rate of pyrene (Pyr) was generally the highest (33.3–66.7%), and the carcinogenic risk of benzo(a)anthracene (BaA) was as high as 14.2–61.1%. The characteristic ratio analysis indicated that petroleum, coal, and biomass combustion were primary sources of PAHs in TCMs. The health risk of TCMs was evaluated using the carcinogenic toxicity contribution rate analysis.

摘要

快速高效的改良QuEChERS方法与高效液相色谱-紫外荧光检测（HPLC-UV-FLD）相结合，可测定和评估74种中药（TCM）中的16种多环芳烃（PAH）。基于样品的QuEChERS方法预处理技术用于通过对植物中药的五个不同药用部分进行脱色和纯化来提高灵敏度和选择性。在干中药中，检测限（LOD）为0.03至0.60μg/ kg。所有线性系数（r ²）的PAHs在0.1至1000 ng / mL的线性范围内超过0.999。五种基质的加标回收率在84.1–98.8％范围内。相对标准偏差（RSD）为1.2％至8.3％。在五份干燥基质中进行了加标回收率测试，以最终验证该方法的准确性。多环芳烃的总含量为1.4至156.0μg/ kg。在16种PAH中，generally（Pyr）的检出率通常最高（33.3–66.7％），苯并（a）蒽（BaA）的致癌风险高达14.2–61.1％。特征比分析表明，石油，煤炭和生物质燃烧是中药中多环芳烃的主要来源。使用致癌毒性贡献率分析评估中医的健康风险。