ABSTRACT

Titanium (Ti) is a widely used biomaterial for dental implants because of its outstanding biocompatibility for hard tissues. Osseointegration, the interaction between implanted biomaterials and living cells in bone, is essential for successful implantation. Rosmarinic acid (RA) is a plant-derived phytochemical with low toxicity and side effects and has various effects that can be applied as a therapeutic substance. The MC3T3-E1 osteoblastic cells on the Ti surface in medium with or without 14 μg/ml RA were used to test RA effects on osteoblast differentiation, cell viability and mineralization during differentiation. RA treatment increased osteoblast differentiation, cell viability and mineralization in MC3T3-E1 osteoblastic cells on Ti surface during differentiation, upregulating Runx-2 and OPG, but downregulating RANKL. This study suggest that RA should be applied as an effective functional and therapeutic substance to enhance osseointegration of osteoblast cells by increasing differentiation, mineralization, and bone formation through the RANKL/RANK/OPG pathway during the differentiation in MC3T3-E1 osteoblastic cells on the Ti surface.

摘要

钛（Ti）因其对硬组织的出色生物相容性而被广泛用于牙种植体。骨整合是植入的生物材料与骨骼中活细胞之间的相互作用，对于成功植入至关重要。迷迭香酸（RA）是一种植物来源的植物化学物质，具有低毒性和副作用，并具有可用作治疗物质的多种作用。在有或没有14μg/ ml RA的培养基中，Ti表面上的MC3T3-E1成骨细胞用于测试RA对分化过程中成骨细胞分化，细胞活力和矿化的影响。RA处理可提高分化过程中Ti表面MC3T3-E1成骨细胞的成骨细胞分化，细胞活力和矿化作用，上调Runx-2和OPG，但下调RANKL。