There is an increasing need to establish populations of threatened plants in threat-free habitat to prevent species extinction. The amount of genetic diversity in founding plants will influence whether the new population has the capacity to persist and evolve over time, and factors that influence the maintenance of genetic diversity, such as the mating systems, will also play a role in population persistence. We developed 13 nuclear microsatellite markers and used these to evaluate genetic diversity and mating system parameters of three translocated populations of two subspecies of Lambertia orbifolia, and compared these parameters to seven wild populations. Genetic diversity was maintained in the translocated population of L. orbifolia subsp. Scott River Plains, established using a single source population (N_ar = 3.270 and H_e = 0.478 in translocated population; N_ar = 3.280 and H_e = 0.534 in wild populations), and maintained or increased in the two admixed translocated populations of L. orbifolia subsp. orbifolia (N_ar = 3.115, 3.830 and H_e = 0.511, 0.635 in translocated populations; N_ar = 2.708 and H_e = 0.438 in wild populations) compared to wild populations of each subspecies. Mating system parameters were comparable between translocated and wild populations of L. orbifolia subsp. Scott River Plains indicating the likelihood of genetic diversity being maintained in future generations. However, there was increased selfing in translocated populations of L. orbifolia subsp. orbifolia, suggesting suboptimal pollination and high values for the inbreeding coefficient in these admixed populations (F_is = 0.474, 0.275), which may be an artifact of the Wahlund effect or from less fit (inbred) seedlings surviving ex situ propagation and translocation.

中文翻译：

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长寿命多年生灌木 Lambertia orbifolia (Proteaceae) 易位成功的遗传和交配系统评估

越来越需要在无威胁的栖息地建立受威胁的植物种群，以防止物种灭绝。创始植物中遗传多样性的数量将影响新种群是否具有持续和随时间进化的能力，而影响遗传多样性维持的因素，如交配系统，也将在种群持久性中发挥作用。我们开发了 13 个核微卫星标记，并使用这些标记来评估Lambertia orbifolia两个亚种的三个易位种群的遗传多样性和交配系统参数，并将这些参数与七个野生种群进行比较。在L. orbifolia易位种群中保持遗传多样性亚种 斯科特河平原，使用单源群建立（Ñ _AR  = 3.270和ħ _Ë 在易位人口= 0.478; ñ _AR  = 3.280和ħ _Ë  = 0.534在野生种群），并保持或在两个混合易位种群增加大号. orbifolia subsp。orbifolia（Ñ _AR  = 3.115，3.830和ħ _ë  = 0.511，0.635在易位人群; ñ _AR  = 2.708和ħ _Ë = 0.438（在野生种群中）与每个亚种的野生种群相比。交配系统参数在L. orbifolia subsp 的易位种群和野生种群之间具有可比性。斯科特河平原表明遗传多样性在后代中得以保持的可能性。然而，在L. orbifolia subsp. 的易位种群中自交增加。o rbifolia，表明这些混合种群中的授粉和近交系数值偏高（F_是 = 0.474, 0.275），这可能是 Wahlund 效应的人工产物，或者来自不合适的（近交）幼苗在异地繁殖和易位中存活下来。