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Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-02-18 , DOI: 10.1002/mbo3.1160
Luis Romero Soto 1, 2 , Habib Thabet 1, 3 , Reuben Maghembe 1, 4 , Denise Gameiro 1 , Doan Van-Thuoc 5 , Tarek Dishisha 6 , Rajni Hatti-Kaul 1
Affiliation  

Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate poly(hydroxyalkanoates) (PHA). In this study, the potential of the bacterium for co‐production of exopolysaccharides (EPS) and PHA was investigated. Genome sequence analysis of the closely related Yangia sp. CCB‐M3 isolated from mangroves in Malaysia revealed genes encoding enzymes participating in different EPS biosynthetic pathways. The effects of various cultivation parameters on the production of EPS and PHA were studied. The highest level of EPS (288 mg/L) was achieved using sucrose and yeast extract with 5% NaCl and 120 mM phosphate salts but with modest PHA accumulation (32% of cell dry weight, 1.3 g/L). Growth on fructose yielded the highest PHA concentration (85% of CDW, 3.3 g/L) at 90 mM phosphate and higher molecular weight EPS at 251 mg/L yield at 120 mM phosphate concentration. Analysis of EPS showed a predominance of glucose, followed by fructose and galactose, and minor amounts of acidic sugars.

中文翻译:

中度嗜盐菌 Yangia sp. 的代谢潜力。ND199 用于联合生产聚羟基链烷酸酯和胞外多糖

Yangia sp. ND199 是一种从越南北部红树林样本中分离出来的中度嗜盐细菌,早先有报道称这种细菌在几种糖和甘油上生长以积累聚(羟基链烷酸酯)(PHA)。在这项研究中,研究了细菌共同生产胞外多糖 (EPS) 和 PHA 的潜力。密切相关的Yangia的基因组序列分析sp. 从马来西亚红树林中分离的 CCB-M3 揭示了编码参与不同 EPS 生物合成途径的酶的基因。研究了各种栽培参数对EPS和PHA产量的影响。使用含 5% NaCl 和 120 mM 磷酸盐的蔗糖和酵母提取物获得最高水平的 EPS (288 mg/L),但具有适度的 PHA 积累(细胞干重的 32%,1.3 g/L)。在果糖上的生长在 90 mM 磷酸盐下产生最高的 PHA 浓度(CDW 的 85%,3.3 g/L),在 120 mM 磷酸盐浓度下产生更高分子量的 EPS 251 mg/L。EPS 的分析显示葡萄糖占优势,其次是果糖和半乳糖,以及少量的酸性糖。
更新日期:2021-02-19
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