Environmental exposure to arsenite (As⁺³) is known to induce immunotoxicity. Natural killer (NK) cells are innate lymphoid cells act as professional killers of tumor cells. Our previous report indicated that 500 ppb As⁺³ drinking water exposure induced significant DNA damage in the NK cells of C57BL/6 mice. Myricetin is a plant-derived flavonoid known as a strong antioxidant. In this study, daily administration of myricetin at 20 mg/kg was found to alleviate the cell population decrease and DNA damage in the NK cells of BALB/c mice exposed to 500 and 1000 ppb As⁺³ via drinking water. Oxidative stress and poly(ADP-ribose) polymerase 1 (PARP-1) inhibition were induced by As⁺³ at 1 and 2 μM in isolated mouse NK cells in vitro, which were attenuated by 20 μM myricetin. The mitigatory effect of myricetin on the PARP-1 inhibition in NK cells treated with As⁺³ was also found to be the result of its prevention of the zinc loss induced by As⁺³ on PARP-1. Collectively, these results demonstrated, for the first time, that myricetin could protect NK cells from As⁺³ induced DNA through attenuating oxidative stress and retaining PARP-1 activity, indicating that myricetin may be utilized for the prevention of the immunotoxicity induced by As⁺³ in NK cells.

已知环境暴露于亚砷酸盐 (As ⁺³ ) 会诱发免疫毒性。自然杀伤 (NK) 细胞是先天淋巴细胞，充当肿瘤细胞的专业杀手。我们之前的报告表明，500 ppb As ⁺³饮用水暴露在 C57BL/6 小鼠的 NK 细胞中诱导了显着的 DNA 损伤。杨梅素是一种植物来源的黄酮类化合物，被称为强抗氧化剂。在这项研究中，发现每天以 20 mg/kg 的剂量施用杨梅素可以缓解通过饮用水暴露于 500 和 1000 ppb As ⁺³的 BALB/c 小鼠 NK 细胞的细胞数量减少和 DNA 损伤。在分离的小鼠 NK 细胞中，1 μM 和 2 μM的 As ⁺³诱导氧化应激和聚（ADP-核糖）聚合酶 1 (PARP-1) 抑制体外，其被 20 μM 杨梅素减弱。杨梅素对用 As ⁺³处理的 NK 细胞中 PARP-1 抑制的缓解作用也被发现是其预防由 As ⁺³对 PARP-1诱导的锌损失的结果。总的来说，这些结果首次表明，杨梅素可以通过减弱氧化应激和保留 PARP-1 活性来保护 NK 细胞免受 As ⁺³诱导的 DNA 的影响，表明杨梅素可用于预防 As ⁺诱导的免疫毒性。³在 NK 细胞中。