In this study, we have compared time series of pollen concentration originating from two Hirst-type volumetric samplers that were equipped with different sampling heads. To collect airborne pollen, we have used classic sampler with mobile sampling head including wind vane and adapted sampler with fixed sampling head having two-layered inlet, like in Sigma-2 passive sampler. The devices were placed at the roof level, on the top of the building of the Faculty of Sciences located in Novi Sad, Serbia. The sampling of airborne pollen was performed from February until October 2019. Along with the pollen data, meteorological conditions were recorded with an automated weather station measuring solar radiation, air temperature, relative humidity, wind speed and precipitation. Time series of daily pollen concentrations expressed high correlations, although there were large differences on the hourly basis. Absolute difference between hourly values showed very weak correlation with relevant meteorological parameters: temperature, humidity, precipitation, wind speed and turbulent kinetic energy, leading to the conclusion that sampling with both heads was not affected by meteorological conditions. Counting the pollen grains from the whole sample and not just from 10% of the area, which is the minimum requirement, was done for the six days in the season and proved that error introduced by subsampling during analysis was the main reason for differences in time series. To conclude, replacing mobile sampling head with fixed sampling head having two-layered inlet does not notably affect the quantity of pollen recorded by the Hirst-type volumetric method.

在这项研究中，我们比较了两个装有不同采样头的Hirst型体积采样器产生的花粉浓度的时间序列。为了收集空气中的花粉，我们使用了经典的采样器，包括带有风向标的可移动采样头，以及带有固定采样头的自适应采样器，该采样器具有两层入口，就像Sigma-2无源采样器一样。这些设备被放置在位于塞尔维亚诺维萨德的科学学院大楼顶部的屋顶上。空气中花粉的采样从2月至2019年10月进行。除花粉数据外，还通过自动气象站记录了气象条件，该气象站测量了太阳辐射，空气温度，相对湿度，风速和降水。每日花粉浓度的时间序列表现出高度相关性，尽管每小时的时间差异很大。每小时值之间的绝对差与温度，湿度，降水，风速和湍动能等相关气象参数之间的相关性非常弱，从而得出结论，双头采样不受气象条件的影响。在整个季节的六天内，对整个样品中的花粉粒进行计数，而不仅仅是从最小面积的10％开始计数，这证明了在分析过程中通过二次采样引入的误差是时间差异的主要原因。系列。总之，用具有两层入口的固定采样头代替移动采样头不会显着影响通过Hirst型体积法记录的花粉量。