There have been several studies carried out to irradiate Helminthiasis however very little research have been carried out where in the enzymatic activity of plants are exploited to antagonize infections. Here we are analyzing the anthelmintic activity of Cinnamomum cappara leaf extract against Pheretima posthuma complimented by proteolytic action. The fresh leaves of Cinnamomum cappara was collected from local areas of Thrissur during December 2019. Plants were identified and authenticated by morphological and molecular characterization. The enzymatic action was analyzed by plotting Lineweaver–Burk plot which suggested that the extract possess the Km 185.77 μM for casein as substrate and obeyed Michaelis–Menten kinetics with typical hyperbolic relation with enzyme and increasing concentration of substrate. The effect of extract upon study subject was in directly proportional with concentration of antagonist where higher activities were obtained in high concentrations. The anatomical and histological studies suggested that the activity of extract was due to the degradation of muscular bundle of subject that resulted in the leakage of ceolomic fluid. Cinnamomum cappara leaf extract possessed high degree of protease intervened anthelmintic activity against Pheretima posthuma. As the study subject show immense morphological and physiological resemblance with all other helminthic parasites, this results shall be adopted to further clinical and pharmacological applications.

中文翻译：

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蛋白水解酶仲裁蠕虫病拮抗由樟cappara在叶提取物地龙posthuma

已经进行了数种辐照蠕虫病的研究，但是很少进行利用植物的酶活性来拮抗感染的研究。在这里，我们正在分析桂皮叶提取物的抗驱虫活性，该蛋白具有蛋白水解作用。肉桂的新鲜叶子是在2019年12月从Thrissur的当地地区收集的。通过形态和分子特征鉴定并鉴定了植物。通过绘制Lineweaver-Burk图来分析酶的作用，该图表明该提取物以酪蛋白为底物具有Km 185.77μM，并遵循Michaelis-Menten动力学，与酶呈典型的双曲线关系，底物浓度增加。提取物对研究对象的作用与拮抗剂的浓度成正比，在高浓度下获得更高的活性。解剖学和组织学研究表明，提取物的活性是由于受试者肌肉束的降解，导致了脑液的渗漏。肉桂叶提取物具有高水平的蛋白酶干预驱虫活性。由于研究对象显示出与所有其他蠕虫寄生虫的巨大形态和生理相似性，因此该结果应被用于进一步的临床和药理应用。解剖学和组织学研究表明，提取物的活性是由于受试者的肌肉束的降解，导致了体液的泄漏。肉桂叶提取物具有高水平的蛋白酶干预驱虫活性。由于研究对象显示出与所有其他蠕虫寄生虫的巨大形态和生理相似性，因此该结果应被进一步用于临床和药理应用。解剖学和组织学研究表明，提取物的活性是由于受试者肌肉束的降解，导致了脑液的渗漏。肉桂叶提取物具有高水平的蛋白酶干预驱虫活性。由于研究对象显示出与所有其他蠕虫寄生虫的巨大形态和生理相似性，因此该结果应被用于进一步的临床和药理应用。