The application of excessive chemical fertilizers during the cultivation of Astragalus membranaceus leads to a decline in the quality of this medicinal plant as well as the soil’s sustainable productivity. In this study, we developed a special microbial organic fertilizer for A. membranaceus and investigated its effects on plant growth and rhizosphere microbial communities. The root biomass and main active components of A. membranaceus in different growth stages were measured to assess the impacts of microbial organic manure on plant growth. Meanwhile, 16S rRNA and ITS1 amplicons were amplified and high-throughput sequencing was performed to detect the dynamic impacts of microbial organic manure on rhizosphere microbial communities. The results demonstrated that microbial organic manure significantly increased wet and dry weights of A. membranaceus seedlings and the accumulation of two effective components (flavonoids and saponin) in bacterial fertilizer treatment groups are significant higher than the control group. Research on rhizosphere microbial flora shows that the number and polymorphism of bacteria and fungi were decreased after the application of special fertilizer during the rapid growth period of plant and then gradually increased with seedling growth. The community structure of bacteria was regulated after the application of special fertilizer, and the beneficial bacteria for plant growth are enriched. Functional profiles prediction showed that significant shifts in metabolic functions impacting KEGG pathways of the microbial fertilizer treatment groups are related to metabolism and biosynthesis. The results indicate that the microbial organic manure can improve A. membranaceus growth by providing appropriate nutrients and regulating the rhizosphere microbial community which has good potential in ecological cultivation of A. membranaceus.

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微生物有机肥对黄芪生长和根际微生物群落的影响

在黄芪的栽培过程中使用过量的化学肥料会导致这种药用植物的质量下降以及土壤的可持续生产力下降。在这项研究中，我们开发了一种用于膜曲霉的特殊微生物有机肥料，并研究了其对植物生长和根际微生物群落的影响。测量了不同生长阶段膜农杆菌的根生物量和主要活性成分，以评估微生物有机肥对植物生长的影响。同时，扩增了16S rRNA和ITS1扩增子，并进行了高通量测序，以检测微生物有机肥对根际微生物群落的动态影响。结果表明，微生物有机肥显着增加了A的湿重和干重。细菌肥料处理组的膜生苗和两种有效成分（类黄酮和皂苷）的积累均显着高于对照组。对根际微生物菌群的研究表明，在植物的快速生长时期，施用特殊肥料后细菌和真菌的数量和多态性降低，然后随着幼苗的生长逐渐增加。施用特殊肥料后，细菌的群落结构得到了调节，丰富了植物生长的有益细菌。功能概况预测表明，影响微生物肥料治疗组的KEGG途径的代谢功能的显着变化与代谢和生物合成有关。结果表明，微生物有机肥可以改善A。