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Trans-Cinnamaldehyde Increases Random Pattern Flap Survival Through Activation of the Nitric Oxide Pathway
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2021-02-18 , DOI: 10.2147/dddt.s297458 Xiaobin Luo 1 , Bin Zhao 2 , Baoxia Chen 2 , Hongyu Chen 1 , Tao Han 1 , Najeeb Bassam Najeeb Bsoul 1 , Hede Yan 1
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2021-02-18 , DOI: 10.2147/dddt.s297458 Xiaobin Luo 1 , Bin Zhao 2 , Baoxia Chen 2 , Hongyu Chen 1 , Tao Han 1 , Najeeb Bassam Najeeb Bsoul 1 , Hede Yan 1
Affiliation
Background: The application of random pattern skin flaps is limited in plastic surgery reconstruction due to necrosis. Trans-cinnamaldehyde has antibacterial, anticancer, and antioxidant properties. In this study, we aimed to investigate the effect of trans-cinnamaldehyde on skin flap survival and its possible mechanism regarding nitric oxide.
Materials and Methods: One hundred forty male Sprague-Dawley rats were randomly divided into seven groups (n = 20 each group). After the dorsal flap was raised, different doses of trans-cinnamaldehyde (10, 20, and 30 mg/kg) were immediately given by oral gavage in the three different groups. To assess the possible involvement of the nitric oxide system, NG-nitro-L-arginine methyl ester (L-NAME, a nonselective nitric oxide synthase inhibitor) was used in this study. All flap samples were incised on postoperative day 7.
Results: Our results showed that flap survival was increased significantly in the 20 mg/kg (P < 0.001) trans-cinnamaldehyde (TC) group compared to the control group or 30 mg/kg TC group. This protective function was restrained by coadministration of L-NAME with 20 mg/kg TC. The results of histopathology, laser Doppler, arteriography mediated with oxide–gelatine, and fluorescent staining all showed a significant increase in capillary count, collagen deposition, angiogenesis, and flap perfusion. Immunohistochemistry results revealed a significant increase in the expression of CD34, eNOS, and VEGF.
Conclusion: Trans-cinnamaldehyde increased flap survival through the nitric oxide synthase pathway and contributed to angiogenesis. A concentration of 20 mg/kg trans-cinnamaldehyde was recommended in this study.
中文翻译：
反式肉桂醛通过激活一氧化氮通路增加随机模式皮瓣的存活率
背景：由于坏死，随机图案皮瓣在整形外科重建中的应用受到限制。反式肉桂醛具有抗菌、抗癌和抗氧化的特性。在这项研究中，我们旨在研究反式肉桂醛对皮瓣存活的影响及其与一氧化氮有关的可能机制。
材料与方法： 140 只雄性 Sprague-Dawley 大鼠随机分为 7 组（每组 20 只）。掀起背侧皮瓣后，三组立即经口灌胃给予不同剂量的反式肉桂醛（10、20、30 mg/kg）。为了评估一氧化氮系统的可能参与，NG本研究使用-硝基-L-精氨酸甲酯（L-NAME，一种非选择性一氧化氮合酶抑制剂）。所有皮瓣样本均在术后第 7 天切开。
结果：我们的结果显示，与对照组或 30 mg/kg TC 相比，20 mg/kg (P < 0.001) 反式肉桂醛 (TC) 组的皮瓣存活率显着增加团体。这种保护功能受到 L-NAME 与 20 mg/kg TC 的共同给药的限制。组织病理学、激光多普勒、氧化明胶介导的动脉造影和荧光染色的结果均显示毛细血管计数、胶原沉积、血管生成和皮瓣灌注显着增加。免疫组织化学结果显示 CD34、eNOS 和 VEGF 的表达显着增加。
结论：反式肉桂醛通过一氧化氮合酶途径增加皮瓣存活率并促进血管生成。本研究建议使用 20 mg/kg 反式肉桂醛的浓度。
更新日期：2021-04-20
Materials and Methods: One hundred forty male Sprague-Dawley rats were randomly divided into seven groups (n = 20 each group). After the dorsal flap was raised, different doses of trans-cinnamaldehyde (10, 20, and 30 mg/kg) were immediately given by oral gavage in the three different groups. To assess the possible involvement of the nitric oxide system, NG-nitro-L-arginine methyl ester (L-NAME, a nonselective nitric oxide synthase inhibitor) was used in this study. All flap samples were incised on postoperative day 7.
Results: Our results showed that flap survival was increased significantly in the 20 mg/kg (P < 0.001) trans-cinnamaldehyde (TC) group compared to the control group or 30 mg/kg TC group. This protective function was restrained by coadministration of L-NAME with 20 mg/kg TC. The results of histopathology, laser Doppler, arteriography mediated with oxide–gelatine, and fluorescent staining all showed a significant increase in capillary count, collagen deposition, angiogenesis, and flap perfusion. Immunohistochemistry results revealed a significant increase in the expression of CD34, eNOS, and VEGF.
Conclusion: Trans-cinnamaldehyde increased flap survival through the nitric oxide synthase pathway and contributed to angiogenesis. A concentration of 20 mg/kg trans-cinnamaldehyde was recommended in this study.
中文翻译：
反式肉桂醛通过激活一氧化氮通路增加随机模式皮瓣的存活率
背景：由于坏死，随机图案皮瓣在整形外科重建中的应用受到限制。反式肉桂醛具有抗菌、抗癌和抗氧化的特性。在这项研究中，我们旨在研究反式肉桂醛对皮瓣存活的影响及其与一氧化氮有关的可能机制。
材料与方法： 140 只雄性 Sprague-Dawley 大鼠随机分为 7 组（每组 20 只）。掀起背侧皮瓣后，三组立即经口灌胃给予不同剂量的反式肉桂醛（10、20、30 mg/kg）。为了评估一氧化氮系统的可能参与，NG本研究使用-硝基-L-精氨酸甲酯（L-NAME，一种非选择性一氧化氮合酶抑制剂）。所有皮瓣样本均在术后第 7 天切开。
结果：我们的结果显示，与对照组或 30 mg/kg TC 相比，20 mg/kg (P < 0.001) 反式肉桂醛 (TC) 组的皮瓣存活率显着增加团体。这种保护功能受到 L-NAME 与 20 mg/kg TC 的共同给药的限制。组织病理学、激光多普勒、氧化明胶介导的动脉造影和荧光染色的结果均显示毛细血管计数、胶原沉积、血管生成和皮瓣灌注显着增加。免疫组织化学结果显示 CD34、eNOS 和 VEGF 的表达显着增加。
结论：反式肉桂醛通过一氧化氮合酶途径增加皮瓣存活率并促进血管生成。本研究建议使用 20 mg/kg 反式肉桂醛的浓度。
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