A metal oxide affinity probe derived from MIL-125 for selective enrichment of endogenous phosphopeptides,Analyst

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A metal oxide affinity probe derived from MIL-125 for selective enrichment of endogenous phosphopeptides
Analyst ( IF 3.6 ) Pub Date : 2021-1-19 , DOI: 10.1039/d0an02174a
Yameng Zhao ₁ , Lingyi Zhang , Licheng Cao , Lei Zhang , Weibing Zhang

Affiliation

Highly effective enrichment of endogenous phosphopeptides from complex biological samples is an essential and crucial theme in the analysis of phosphopeptidomics. Herein, an ordered mesoporous TiO₂/C composite (denoted as Ti–MCM) was prepared by the pyrolysis of MIL-125 under a N₂ atmosphere. The obtained Ti–MCM possesses a high specific surface area (165 m² g⁻¹), a uniform pore size (3.75 nm), and a large amount of Ti (46%). By utilizing the selective chelation between Ti–MCM and phosphopeptides, 25 phosphopeptides were detected in α-casein digest after enrichment. The material shows good selectivity even in the presence of 2000-fold excess of interference peptides. It was also used to enrich endogenous phosphopeptides from the complex samples of human serum and saliva and showed a good performance.

中文翻译：

一种源自 MIL-125 的金属氧化物亲和探针，用于选择性富集内源性磷酸肽

从复杂的生物样品中高效富集内源性磷酸肽是磷酸肽组学分析中的一个重要和关键的主题。_{在此，通过 MIL-125 在 N 2}气氛下的热解制备有序介孔 TiO ₂ /C 复合材料（表示为 Ti-MCM）。获得的 Ti-MCM 具有高比表面积 (165 m ² g ^-1), 均匀的孔径 (3.75 nm), 和大量的 Ti (46%)。利用 Ti-MCM 和磷酸肽之间的选择性螯合，富集后在 α-酪蛋白消化物中检测到 25 种磷酸肽。即使存在 2000 倍过量的干扰肽，该材料也显示出良好的选择性。它还用于从人血清和唾液的复杂样品中富集内源性磷酸肽，并表现出良好的性能。

更新日期：2021-02-18

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