Objective. Candida glabrata (C. glabrata) causes infections associated with severe sepsis and high mortality. This study describes the effects of micafungin (MCF), itraconazole (ICZ), and amphotericin B (AmB) on the function of macrophages during C. glabrata infection. Methods. RAW264.1 macrophages were treated with MCF, ICZ, or AmB and then challenged with C. glabrata. Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis. Results. The activity of SOD was significantly increased in RAW264.1 cells that phagocytized C. glabrata and reached a peak level at 6 hours (). ICZ and AmB did not affect the SOD activity in cells that phagocytized C. glabrata versus that in untreated macrophage. C. glabrata stimulated macrophages to secrete cytokines. Neither ICZ nor AmB affected the secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), or tumor necrosis factor-α (TNF-α) by C. glabrata-infected macrophages. However, MCF downregulated the secretion of TNF-α by infected macrophages and reduced the SOD activity of C. glabrata compared with those in untreated controls. Conclusion. Echinocandins may increase their antifungal efficacy by altering the innate immune response of macrophages and attenuating antioxidants of this organism.

中文翻译：

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抗真菌治疗宿主巨噬细胞对光滑念珠菌的影响

客观。光滑念珠菌( C. glabrata ) 引起与严重败血症和高死亡率相关的感染。本研究描述了米卡芬净 (MCF)、伊曲康唑 (ICZ) 和两性霉素 B (AmB) 在光滑念珠菌感染期间对巨噬细胞功能的影响。方法。RAW264.1 巨噬细胞用 MCF、ICZ 或 AmB 处理，然后用C. glabrata攻击。在吞噬作用后的不同时间点测量来自感染巨噬细胞上清液的细胞因子和巨噬细胞中超氧化物歧化酶 (SOD) 的水平。结果。吞噬光滑念珠菌的RAW264.1细胞SOD活性显着增加并在 6 小时达到峰值（）。ICZ 和 AmB 不影响吞噬C. glabrata的细胞与未处理的巨噬细胞中的 SOD 活性。C. glabrata刺激巨噬细胞分泌细胞因子。ICZ 和 AmB 均不影响C. glabrata感染的巨噬细胞分泌白细胞介素 6 (IL-6)、白细胞介素 8 (IL-8) 或肿瘤坏死因子-α (TNF -α )然而，与未处理的对照组相比，MCF 下调受感染巨噬细胞的 TNF -α分泌，并降低C. glabrata的 SOD 活性。结论. 棘白菌素可以通过改变巨噬细胞的先天免疫反应和减弱这种生物体的抗氧化剂来增加其抗真菌功效。