In vivo imaging of human retinal pigment epithelial (RPE) cells has been demonstrated through multiple adaptive optics (AO)-based modalities. However, whether consistent and complete information regarding the cellular structure of the RPE mosaic is obtained across these modalities remains uncertain due to limited comparisons performed in the same eye. Here, an imaging platform combining multimodal AO-scanning light ophthalmoscopy (AO-SLO) with AO-optical coherence tomography (AO-OCT) is developed to make a side-by-side comparison of the same RPE cells imaged across four modalities: AO-darkfield, AO-enhanced indocyanine green (AO-ICG), AO-infrared autofluorescence (AO-IRAF), and AO-OCT. Co-registered images were acquired in five subjects, including one patient with choroideremia. Multimodal imaging provided multiple perspectives of the RPE mosaic that were used to explore variations in RPE cell contrast in a subject-, location-, and even cell-dependent manner. Estimated cell-to-cell spacing and density were found to be consistent both across modalities and with normative data. Multimodal images from a patient with choroideremia illustrate the benefit of using multiple modalities to infer the cellular structure of the RPE mosaic in an affected eye, in which disruptions to the RPE mosaic may locally alter the signal strength, visibility of individual RPE cells, or even source of contrast in unpredictable ways.

中文翻译：

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集成自适应光学元件-SLO和OCT以实现人视网膜色素上皮镶嵌的多模式可视化

已通过多种基于自适应光学（AO）的方式对人视网膜色素上皮（RPE）细胞进行了体内成像。但是，由于在同一只眼中进行的比较有限，因此不确定是否可以通过这些方式获得有关RPE镶嵌细胞结构的一致且完整的信息。在这里，开发了一种将多模式AO扫描光学检眼镜（AO-SLO）与AO光学相干断层扫描（AO-OCT）相结合的成像平台，以对通过以下四种模式成像的相同RPE细胞进行并排比较： -darkfield，AO增强的吲哚菁绿（AO-ICG），AO红外自发荧光（AO-IRAF）和AO-OCT。在五名受试者中获得了共同注册的图像，其中包括一名脉络膜炎患者。多模态成像提供了RPE镶嵌的多个透视图，这些透视图用于以受检者，位置甚至细胞依赖的方式探索RPE细胞对比度的变化。发现估计的细胞间间距和密度在所有模式之间均与标准数据一致。来自脉络膜炎患者的多峰图像显示了使用多种模式来推断患眼中RPE镶嵌的细胞结构的好处，其中对RPE镶嵌的破坏可能会局部改变信号强度，单个RPE细胞的可见度，甚至对比来源无法预测。发现估计的细胞间间距和密度在所有模式之间均与标准数据一致。来自脉络膜炎患者的多峰图像显示了使用多种模式来推断患眼中RPE镶嵌的细胞结构的好处，其中对RPE镶嵌的破坏可能会局部改变信号强度，单个RPE细胞的可见度，甚至对比来源无法预测。发现估计的细胞间间距和密度在所有模式之间均与标准数据一致。来自脉络膜炎患者的多峰图像显示了使用多种模式来推断患眼中RPE镶嵌的细胞结构的好处，其中对RPE镶嵌的破坏可能会局部改变信号强度，单个RPE细胞的可见度，甚至对比来源无法预测。