Nanoporous Anodic Alumina-Based Sensor for miR-99a-5p Detection as an Effective Early Breast Cancer Diagnostic Tool,ACS Sensors

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Nanoporous Anodic Alumina-Based Sensor for miR-99a-5p Detection as an Effective Early Breast Cancer Diagnostic Tool
ACS Sensors ( IF 8.2 ) Pub Date : 2021-02-18 , DOI: 10.1021/acssensors.0c02222
Iris Garrido-Cano _{1,

2} , Luis Pla _{2,

3,

4} , Sara Santiago-Felipe _{2,

3,

4} , Soraya Simón _{1,

5} , Belen Ortega _{1,

5} , Begoña Bermejo _{1,

5} , Ana Lluch _{1,

5,

6,

7} , Juan Miguel Cejalvo _{1,

5,

6} , Pilar Eroles _{1,

6,

8} , Ramón Martínez-Máñez _{2,

3,

4,

9}

Affiliation

Circulating microRNAs have emerged as potential diagnostic biomarkers. The deregulation of the microRNA miR-99a-5p has been previously described as an effective biomarker of early breast cancer. Herein, we present a new nanoporous anodic alumina (NAA)-based biosensor that can detect plasma miR-99a-5p with high sensitivity and selectivity. NAA pores are loaded with rhodamine B and capped with a specific oligonucleotide that is able to block cargo release until the target is present. In the presence of miR-99a-5p, the capping oligonucleotide recognizes the miR-99a-5p sequence and displaces it allowing the release of the encapsulated dye. This method is able to successfully distinguish healthy controls from breast cancer patients, even at early stages with high efficiency, showing the presented system as a promising tool for breast cancer detection.

中文翻译：

用于检测 miR-99a-5p 的纳米多孔阳极氧化铝传感器作为一种有效的早期乳腺癌诊断工具

循环 microRNA 已成为潜在的诊断生物标志物。先前已将 microRNA miR-99a-5p 的失调描述为早期乳腺癌的有效生物标志物。在此，我们提出了一种新的基于纳米多孔阳极氧化铝 (NAA) 的生物传感器，可以高灵敏度和选择性地检测血浆 miR-99a-5p。NAA 孔中装有罗丹明 B，并用一种特定的寡核苷酸加帽，该寡核苷酸能够阻止货物释放，直到目标存在。在 miR-99a-5p 存在的情况下，加帽寡核苷酸识别 miR-99a-5p 序列并取代它，从而释放封装的染料。该方法能够成功地将健康对照与乳腺癌患者区分开来，即使在早期阶段也具有很高的效率，表明所提出的系统是一种很有前途的乳腺癌检测工具。

更新日期：2021-03-26

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