ABSTRACT

Knowledge about fertiliser limitation to soil microbes is crucial for exploring microbial community succession and ecosystem functions. In this study, an experiment was conducted to promote ecological restoration by different treatments of organic fertiliser (OF), microbial fertiliser (MF) and non-fertiliser (CK) in a coal mine dump. We investigated microbial community structure using high-throughput sequencing. Soils under OF and MF treatment showed distinct community structures with highly bacterial α-diversity due to the variation of soil pH and organic matters (SOM). Of those, the community dissimilarity between OF and CK was higher. The DistLM analysis indicated that SOM and available nitrogen (AN) significantly influenced microbial community structure. Furthermore, functional annotation by the FAPROTAX revealed that the functional groups associating with C, N, and S cycling increased significantly under OF and MF treatment, but decreased that associated with parasites or symbionts. Compared with MF, the functional groups with OF treatment varied greatly. However, the function groups including nitrogen_fixation, hydrocarbon_degradation and methanotrophy were significantly improved under the MF treatment, which have an important implication for soil quality and microbial growth. It was concluded that MF treatment could improve microbial community structure and enhance potential ecosystem function in coal mine dump soils.

摘要

关于肥料对土壤微生物的限制的知识对于探索微生物群落演替和生态系统功能至关重要。在这项研究中，进行了一项实验，通过对矿场中的有机肥（OF），微生物肥料（MF）和非肥料（CK）进行不同处理来促进生态恢复。我们使用高通量测序研究了微生物群落结构。由于土壤pH和有机物（SOM）的变化，经过OF和MF处理的土壤表现出独特的群落结构，具有高度的细菌α多样性。其中，OF和CK之间的群体相似性更高。DistLM分析表明，SOM和有效氮（AN）显着影响微生物群落结构。此外，FAPROTAX的功能注释显示，在OF和MF处理下，与C，N和S循环相关的功能基团显着增加，但与寄生虫或共生体相关的功能基团减少。与MF相比，OF处理的官能团差异很大。然而，在MF处理下，固氮，碳氢降解和甲烷氧化等功能群得到了显着改善，这对土壤质量和微生物生长具有重要意义。得出的结论是，MF处理可以改善煤矿场土壤中的微生物群落结构并增强潜在的生态系统功能。OF处理的功能组差异很大。然而，在MF处理下，固氮，碳氢降解和甲烷氧化等功能组得到了显着改善，这对土壤质量和微生物生长具有重要意义。得出的结论是，MF处理可以改善煤矿场土壤中的微生物群落结构并增强潜在的生态系统功能。OF处理的功能组差异很大。然而，在MF处理下，固氮，碳氢降解和甲烷氧化等功能群得到了显着改善，这对土壤质量和微生物生长具有重要意义。得出的结论是，MF处理可以改善煤矿场土壤中的微生物群落结构并增强潜在的生态系统功能。