Food waste (FW) minimization at the source by using food waste biodigester (FWBs) has a vast potential to lower down the impact of increasing organic fraction in municipal solid waste generation. To this end, this research sought to check the performance of locally isolated hydrolase-producing bacteria (HPB) to improve food waste biodegradation rate. Two under-explored HPB identified as Bacillus paralicheniformis GRA2 and Bacillus velezensis TAP5 were able to produce maximum amylase, cellulase, protease and lipase activities, and demonstrated a significant hydrolase synergy in co-culture fermentation. In vitro biodegradation analysis of both autoclaved and non-autoclaved FW revealed that the HPB inoculation was effective to degrade total solids (>62%), protein (>19%), total fat (>51), total sugar (>86%), reducing sugar (>38%) and starch (>50%) after 8-day incubation. All co-culture treatments were recorded superior to the respective monocultures and the uninoculated control. The results of FW biodegradation using batch-biodigester trial indicated that the 1500 mL and 1000 mL inoculum size of HPB inoculant reached a plateau on the 4th day, with gross biodegradation percentage (GBP) of >85% as compared to control (66.4%). The 1000 mL inoculum was sufficient to achieve the maximum GBP (>90%) of FW after an 8-day biodigestion in a FWB.

通过使用食物残渣生物消化物（FWB）从源头上最大限度地减少食物残渣（FW），具有巨大的潜力来降低城市固体废物产生中有机物含量增加的影响。为此，该研究试图检查局部分离的产生水解酶的细菌（HPB）的性能，以提高食物垃圾的生物降解率。两种未充分开发的HPB被鉴定为副芽孢杆菌GRA2和Velezensis芽孢杆菌TAP5能够产生最大的淀粉酶，纤维素酶，蛋白酶和脂肪酶活性，并在共培养发酵中表现出显着的水解酶协同作用。高压灭菌和非高压灭菌FW的体外生物降解分析表明，接种HPB可以有效降解总固体（> 62％），蛋白质（> 19％），总脂肪（> 51），总糖（> 86％）孵育8天后，还原糖（> 38％）和淀粉（> 50％）。记录的所有共培养处理均优于各自的单培养和未接种的对照。使用分批生物消化器试验进行的FW生物降解的结果表明，HPB接种剂的1500 mL和1000 mL接种量在第4天达到平稳期，总生物降解率（GBP）高于对照（66.4％） 。