Oxidative stress, DNA damage, and unresolved inflammation are the predisposing factors of many chronic and degenerative diseases, including cancer. Stingless bee honey (SBH) is recognized to have high medicinal value by traditional medicine practitioners and has been used to treat various illnesses traditionally. This study aimed to determine the antioxidant, anti-inflammatory, and genoprotective effects of SBH by using in vitro cell culture models. The sugar content, total phenolic content, radical scavenging activity, and ferric reducing antioxidant power (FRAP) of SBH were determined in this study. Then, the protective effect of SBH against hydrogen peroxide (H₂O₂)-induced cell death and DNA damage was studied by using WIL2-NS human lymphoblastoid cell line, while the lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophages cell line was used to study the anti-inflammatory effects of SBH. Results from this present study showed that the major sugar contents of SBH were fructose (19.39 + 0.01%) and glucose (14.03 ± 0.03%). Besides, the total phenolic content, the radical scavenging activity, and the FRAP value of SBH were 15.38 ± 0.02 mg GAE/100 g of honey, 34.04 ± 0.21%, and 206.77 + 1.76 μM AAE/100 g honey respectively. Pretreatment with SBH protected WIL2-NS cells from H₂O₂-induced cell death and DNA damage (p < 0.001). Moreover, SBH was also able to attenuate the production of nitric oxide by inhibiting the expression of inducible nitric oxide synthase in LPS-induced RAW 264.7 cells (p < 0.001). In conclusion, SBH is rich in total phenolic content and possesses strong antioxidant, anti-inflammatory, and genoprotective properties. Our current findings suggest that SBH might be useful in the prevention and treatment of many diseases caused by oxidative stress and inflammation assuming the observed effects are also achievable in vivo.

氧化应激，DNA损伤和未解决的炎症是包括癌症在内的许多慢性和退行性疾病的诱发因素。无刺蜂蜂蜜（SBH）被传统医学从业者认为具有很高的药用价值，并且传统上已用于治疗各种疾病。这项研究旨在通过使用体外细胞培养模型来确定SBH的抗氧化，抗炎和基因保护作用。本研究确定了SBH的糖含量，总酚含量，自由基清除活性和铁还原抗氧化能力（FRAP）。然后，SBH对过氧化氢（H ₂ O ₂通过使用WIL2-NS人淋巴母细胞样细胞系来研究）诱导的细胞死亡和DNA损伤，而脂多糖（LPS）诱导的RAW 264.7鼠巨噬细胞系则用于研究SBH的抗炎作用。本研究的结果表明，SBH的主要糖含量为果糖（19.39 + 0.01％）和葡萄糖（14.03±0.03％）。此外，SBH的总酚含量，自由基清除活性和FRAP值分别为蜂蜜/ 100g蜂蜜15.38±0.02 mg GAE，34.04±0.21％和206.77 + 1.76μMAAE / 100 g蜂蜜。预处理SBH保护WIL2-NS细胞选自H ₂ ö ₂诱导的细胞死亡和DNA损伤（p <0.001）。此外，SBH还能够通过抑制LPS诱导的RAW 264.7细胞中诱导型一氧化氮合酶的表达来减弱一氧化氮的产生（p <0.001）。总之，SBH的总酚含量丰富，并具有强大的抗氧化，抗炎和基因保护特性。我们目前的发现表明，SBH可能在预防和治疗由氧化应激和炎症引起的多种疾病中发挥作用，前提是所观察到的效果在体内也是可以实现的。