Zanthoxylum acanthopodium has several biological activities, such as antimicrobial, anti-inflammatory, and antioxidant properties. This strong antioxidant herb can be used as a drug for hypertension. FasL and cytochrome c expression play roles in the apoptotic pathway in the placenta. This study focused on the histological change in apoptosis via cytochrome c and Fas ligand expression by investigating whether Zanthoxylum acanthopodium (ZA) fruits affect apoptosis. The present study consisted of five treatments: Normal pregnant rats (C), Hypertension rats (C + ), hypertension rats + extra virgin olive oil (EVOO) (T1), Hypertension rats + ZA (T2), and hypertension rats + EVOO + ZA (T3). Hypertension was induced in rats by injecting 3 mlml of 6% NaCl. Nanoherbal of ZA (100 mg/kg BW) and EVOO (1 ml) were given on the 13th–19th days of pregnancy. Pregnant rats were dissected on the 20th day of pregnancy by cervical dislocation. ELISA assays were performed for the analysis of HSP-70 expression. Immunohistochemistry and TUNEL assays were used to analyse the histological changes in placental tissue. The results showed that cytochrome c and FasL protein exposure levels in the labyrinth, basal, and yolk sac zones were increased during hypertensive pregnancy (P < 0.0001) in rats. The administration of nanoherbal of ZA decreased the expression of cytochrome c and FasL. A significant difference was found in the combination of nanoherbal of ZA and EVOO.

花椒花椒具有多种生物学活性，例如抗微生物，抗炎和抗氧化特性。这种强抗氧化剂可以用作高血压药物。FasL和细胞色素c的表达在胎盘的凋亡途径中发挥作用。通过研究花椒棘皮an是否通过细胞色素c和Fas配体表达来研究凋亡的组织学变化。（ZA）水果会影响细胞凋亡。本研究包括五种治疗方法：正常妊娠大鼠（C），高血压大鼠（C +），高血压大鼠+特级初榨橄榄油（EVOO）（T1），高血压大鼠+ ZA（T2）和高血压大鼠+ EVOO + ZA（T3）。通过注射3 ml / l的6％NaCl诱发大鼠高血压。在怀孕的第13-19天给予ZA的纳米草药（100 mg / kg体重）和EVOO（1 ml）。在怀孕的第20天，通过颈脱位解剖怀孕的大鼠。进行ELISA分析以分析HSP-70表达。免疫组织化学和TUNEL法用于分析胎盘组织的组织学变化。结果表明，细胞色素c高血压妊娠期间大鼠的迷宫，基底和卵黄囊区的FasL和FasL蛋白暴露水平增加（P <0.0001）。ZA的纳米草药的施用降低了细胞色素c和FasL的表达。在ZA和EVOO的纳米草药的组合中发现了显着差异。