At present, the effect of ganglioside combined with Jiaji electroacupuncture (Jiaji EA) on SCI still remains unclear. This study explores the effect of ganglioside combined with electroacupuncture on Nogo/NgR signal pathway in spinal cord tissue of spinal cord injury (SCI) rats. Basso Beattie Bresnahan (BBB) score was used to evaluate spinal cord function after modeling and 14 days post ganglioside and electroacupuncture treatment. RT-qPCR and western blot were performed to evaluate the expression levels of targets in spinal cord tissue. After 14 days of treatment, the BBB scores of Jiaji EA group, ganglioside group and combination group were all improved. The expression levels of IL-1β, IL-6 and TNF-α in Jiaji EA group, ganglioside group and combination group were significantly lower than those in model group. Both of mRNA and protein expression levels of Nogo-A, NgR and LINGO-1 in the model group were significantly higher than those in the Jiaji EA group, ganglioside group and combination group. Ganglioside combined with Jiaji EA has a stronger effect on promoting the recovery of nerve function. Its mechanism of action may be related to its inhibition of the expression of proinflammatory cytokines such as IL-1β, IL-6 and TNF-α and Nogo-NgR signal pathway to promote neuronal growth. Our results will provide fundamental information for further SCI studies.

目前，神经节苷脂联合加极电针（加极电针）对SCI的疗效尚不清楚。本研究探讨神经节苷脂联合电针对脊髓损伤（SCI）大鼠脊髓组织中Nogo/NgR信号通路的影响。Basso Beattie Bresnahan (BBB) 评分用于评估建模后和神经节苷脂和电针治疗后 14 天的脊髓功能。进行 RT-qPCR 和蛋白质印迹以评估脊髓组织中靶标的表达水平。治疗14天后，加积电针组、神经节苷脂组和联合组的BBB评分均有所改善。加积电针组、神经节苷脂组和联合组IL-1β、IL-6、TNF-α的表达水平显着低于模型组。模型组Nogo-A、NgR、LINGO-1 mRNA和蛋白表达水平均显着高于加积电针组、神经节苷脂组和联合组。神经节苷脂联合甲极电针对促进神经功能恢复的作用更强。其作用机制可能与其抑制促炎细胞因子如IL-1β、IL-6和TNF-α的表达以及Nogo-NgR信号通路促进神经元生长有关。我们的结果将为进一步的 SCI 研究提供基础信息。其作用机制可能与其抑制促炎细胞因子如IL-1β、IL-6和TNF-α的表达以及Nogo-NgR信号通路促进神经元生长有关。我们的结果将为进一步的 SCI 研究提供基础信息。其作用机制可能与其抑制促炎细胞因子如IL-1β、IL-6和TNF-α的表达以及Nogo-NgR信号通路促进神经元生长有关。我们的结果将为进一步的 SCI 研究提供基础信息。