American foulbrood (AFB) and European foulbrood (EFB) are the two major bacterial diseases affecting honeybees, leading to a decrease in viability of the hive, decreasing honey production, and resulting in significant economic losses to beekeepers. Due to the inefficiency and/or low efficacy of some antibiotics, researches with nanotechnology represent, possibly, new therapeutic strategies. Nanostructure drugs have presented some advantagesover the conventional medicines, such as slow, gradual and controlled release, increased bioavailability, and reduced side-effects. In this study, different infected larvae were collected from two apiaries; the combs that had symptoms of American and European foulbrood were isolated. In vitro antimicrobial activity of camphor tree silver nano-particles against foulbrood diseases were characterized using UV–Vis spectrophotometry and scanning electron microscope (SEM) that proves the formation of silver nanoparticles with size range 160–660 nm. The antimicrobial activity of the silver nanoparticles was tested using agar diffusion assay and proved their ability to effectively cease the pathogenic bacterial growth in both AFB and EFB. DGGE-PCR technique has been applied for the identification of un-common bacterial infections honeybees depending on 16S rRNA amplification from their total extracted DNA and has been identified as Serratia marcescens (TES), deposited in GenBank with a new accession number (MT240613). The results were confirmed strain has been detected by DGGE-PCR analysis causing uniquely infected brood that was attacked by the American Foulbrood It could be concluded that greenly synthesized silver nanoparticles is projected to be used as effective treatment for honeybee bacterial diseases. These material need more investigations under field conditions and study the possibility of its residues in honeybee products such as honey, and beeswax.

美国幼虫腐臭病（AFB）和欧洲幼虫腐臭病（EFB）是影响蜜蜂的两种主要细菌性疾病，导致蜂巢活力下降，蜂蜜产量减少，给养蜂人造成重大经济损失。由于某些抗生素效率低下和/或功效低，纳米技术的研究可能代表了新的治疗策略。与传统药物相比，纳米结构药物具有缓释、渐进、控释、提高生物利用度、减少副作用等优点。在这项研究中，从两个养蜂场收集了不同的感染幼虫；具有美国和欧洲腐臭病症状的鸡冠被隔离。使用紫外-可见分光光度法和扫描电子显微镜（SEM）对樟树银纳米颗粒对幼虫腐臭病的体外抗菌活性进行了表征，证明形成了尺寸范围为160-660 nm的银纳米颗粒。使用琼脂扩散试验测试了银纳米颗粒的抗菌活性，并证明它们能够有效阻止 AFB 和 EFB 中致病菌的生长。 DGGE-PCR 技术已应用于鉴定不常见的细菌感染蜜蜂，依赖于从总提取 DNA 中进行 16S rRNA 扩增，并已被鉴定为粘质沙雷氏菌(TES)，并以新的登录号 (MT240613) 保存在 GenBank 中。结果证实，DGGE-PCR 分析已检测到菌株，导致受到美国臭虫攻击的独特感染蜂巢。可以得出结论，绿色合成的银纳米颗粒预计将用作蜜蜂细菌性疾病的有效治疗方法。 这些材料需要在现场条件下进行更多研究，并研究其在蜂蜜和蜂蜡等蜜蜂产品中残留的可能性。