Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak in rice. To promote disease development, bacteria deliver effectors such as transcription activator-like (TAL) effectors into plant cells to modulate host defense and promote bacterial growth. In this study, we constructed a Tn5 transposon-mediated mutation library of GD41, which possesses 30 TAL effectors. A TalI gene deficient mutant GD21 was obtained. GD21 demonstrated compromised bacterial virulence and growth. Sequence analysis showed that TalI is a typical TAL effector gene with 14.5 repeats and RVDs identical to Tal3 in Xoc BXOR1. Expression of TalI in Xo strains resulted in enhanced bacterial growth in rice and medium. Target gene screening results suggest that TalI does not target the rice gene OsSULTR3;6 and Os09g29100. Orthology analysis showed that TalI does not belong to the 13 conserved TAL ortholog families crossing Xoc strains. Next, the role of TalI in SA, JA, and ET hormone pathways was tested, and our results showed that TalI has an effect on the expression of PR1a, PR1b, and PR5 but not on other marker genes. The HR suppression ability of TalI was then evaluated by introducing avrXa10 and avrXa7 into GD41 and GD21. The transformed strains displayed similar HR eliciting pattern on rice lines IRBB10 and IRBB7, which suggests that TalI does not interfere with effector mediated defense directly.

Xanthomonas oryzae pv。空心稗（XOC）引起水稻细菌性叶条纹。为了促进疾病的发展，细菌将诸如转录激活因子（TAL）效应子等效应子传递到植物细胞中，以调节宿主防御能力并促进细菌生长。在这项研究中，我们构建了Tn5转座子介导的GD41突变文库，该文库具有30个TAL效应子。获得了TalI基因缺陷型突变体GD21。GD21表现出受损的细菌毒力和生长。序列分析表明，塔利与14.5重复与RVDS等同于典型TAL效应基因TAL3在XOC BXOR1。TalI在Xo中的表达菌株导致水稻和培养基中细菌的生长增强。靶基因筛选结果表明TalI不靶向水稻基因OsSULTR3; 6和Os09g29100。正交学分析表明，TalI不属于穿越Xoc菌株的13个保守的TAL直系同源物家族。接下来，测试了TalI在SA，JA和ET激素途径中的作用，我们的结果表明TalI对PR1a，PR1b和PR5的表达有影响，但对其他标记基因没有影响。的HR抑制能力塔利然后通过引入评价avrXa10和avrXa7转换为GD41和GD21。转化的菌株在水稻品系IRBB10和IRBB7上表现出相似的HR诱导模式，这表明TalI不会直接干扰效应子介导的防御。