With the development of tissue engineering, it is no longer a challenge to repair and reconstruct bone defects using bone substitutes. However, in spinal fusion surgery, high rates of fusion failure are difficult to avoid. In our study, we designed a new composite hydrogel and found that it has good osteogenesis and angiogenesis effects. We extracted exosomes produced by rBMSCs (rat bone marrow mesenchymal stem cells) cocultured with the hydrogel to investigate their effects on osteogenesis and angiogenesis.

The results showed that the PG/TCP (PEGMC with β-TCP) promoted rapid osteogenesis, facilitated spinal fusion at a high rate and quality and had an indirect effect on angiogenesis. We found that PG/TCP affected the rBMSC microenvironment, thus changing the function of exosomes; in a further study, we found that PG/TCP-MSC-Exos played a significant role in osteogenesis, which was coupled to angiogenesis. Thus, PG/TCP showed excellent potential in bone regeneration, especially the PG/0.2TCP.

随着组织工程学的发展，使用骨替代物修复和重建骨缺损不再是挑战。但是，在脊柱融合手术中，难以避免高融合失败率。在我们的研究中，我们设计了一种新型复合水凝胶，发现它具有良好的成骨作用和血管生成作用。我们提取了与水凝胶共培养的rBMSC（大鼠骨髓间充质干细胞）产生的外泌体，以研究它们对成骨和血管生成的影响。

结果表明，PG / TCP（带有β-TCP的PEGMC）促进了快速的成骨作用，促进了脊柱融合的高速率和高质量，并间接影响了血管生成。我们发现PG / TCP影响了rBMSC的微环境，从而改变了外泌体的功能。在进一步的研究中，我们发现PG / TCP-MSC-Exos在成骨过程中起着重要作用，这与血管生成有关。因此，PG / TCP在骨骼再生中显示出极好的潜力，尤其是PG / 0.2TCP。