As important members of biothiol species, cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) are involved in numerous physiological activities. Moreover, abnormal levels of these biothiols are closely relevant to a series of diseases. Therefore, it's of great importance and challenges to develop efficient approaches for sensitively detecting biothiols. Herein, a novel fluorescent probe JSC-RSH for sensing Cys/Hcy/GSH was reported. JSC-RSH exhibits excellent properties including red-emitting, high sensitivity and selectivity, as well as rapid response towards biothiols. The probe alone only emits negligible fluorescence, while a remarkable fluorescence emission in the red region could be observed in the presence of biothiols. The calculated limits of detection (LODs) for Cys/Hcy/GSH are 7.9 nM, 10.2 nM and 4.2 nM, respectively. Furthermore, probe JSC-RSH with low cytotoxicity was utilized for monitoring endogenous and exogenous biothiols in A549 cells.

作为生物硫醇物种的重要成员，半胱氨酸（Cys），高半胱氨酸（Hcy）和谷胱甘肽（GSH）参与许多生理活动。此外，这些生物硫醇的异常水平与一系列疾病密切相关。因此，开发有效检测生物硫醇的有效方法具有重大意义和挑战。本文中，报道了用于感测Cys / Hcy / GSH的新型荧光探针JSC-RSH。JSC-RSH具有优异的性能，包括发红光，高灵敏度和高选择性以及对生物硫醇的快速响应。单独的探针仅发出可忽略不计的荧光，而在存在生物硫醇的情况下，可以在红色区域观察到显着的荧光发射。Cys / Hcy / GSH的计算检出限（LOD）分别为7.9 nM，10.2 nM和4.2 nM。此外，具有低细胞毒性的探针JSC-RSH用于监测A549细胞中的内源性和外源性生物硫醇。