Evolutionary immune system upgradation is done by all living organism to survive from the invading pathogens. Insects has a strong defense system comprising of anatomical barrier, humoral antimicrobial peptide (AMP) production and cellular immune components. Locusta migratoria (L. migratoria) is an agricultural pest insect migrating long distance might encounter distinct pathogens. Owing to this, we aimed in identifying AMPs present in the L. migratoria and elucidate the antimicrobial activity. The migratory locust was immunized with Escherichia coli, Staphylococcus aureus, Candida albicans mixture, then RNA was isolated and sequenced. The L. migratoria transcriptome was de novo assembled using trinity and screened for AMPs propensity specifically molecular and aggregation properties. The novelty is tested by blasting in known AMP databases. Finally, obtained novel putative AMPs were then tested for antimicrobial and hemolytic activity. The prediction resulted in 3,524 putative AMPs which was further screened down to ten AMPs for final testing. Two peptides showing promising antimicrobial effects were obtained. All the analyzed peptides showed no hemolysis confirms its non-toxicity. Thus, our peptides could be promising drug candidate and can be used as an alternative to antibacterial or antifungal therapy with further validations.

进化的免疫系统升级是由所有活的生物体完成以从入侵的病原体中存活下来的。昆虫具有强大的防御系统，包括解剖屏障，体液抗菌肽（AMP）产生和细胞免疫成分。Locusta migratoria（L. migratoria）是一种农业害虫，长距离迁移可能会遇到不同的病原体。因此，我们旨在鉴定存在于偏头痛乳杆菌中的AMP，并阐明其抗菌活性。用大肠杆菌，金黄色葡萄球菌，白色念珠菌混合物免疫蝗虫，然后分离RNA并测序。该L.飞蝗使用三位一体从头组装转录组，并筛选AMPs倾向的分子和聚集特性。通过在已知的AMP数据库中进行爆破测试新颖性。最后，然后测试获得的新型推定AMP的抗菌和溶血活性。预测结果得出了3,524个假定的AMP，并进一步筛选了10个AMP进行最终测试。获得了显示出有希望的抗微生物作用的两种肽。所有分析的肽均未显示溶血现象，证实其无毒。因此，我们的肽可能是有前途的候选药物，并且可以通过进一步的验证用作抗菌或抗真菌治疗的替代品。