The mitochondrial genome (mitogenome) has been extensively used in phylogenetics and species-level evolutionary investigations. The lepidopteran family Tortricidae (leaf-roller moths), including the genus Grapholita, contains numerous species of economic importance, but for the majority of Grapholita species, their mitogenomes remain poorly studied. Here, we sequence and annotate the full mitogenome of Grapholita delineana, an important pest of hemp worldwide and compare it with the mitogenomes of two congeneric species available from GenBank. The G. delineana mitogenome is 15,599 bp long, including 37 typical mitochondrial genes and an A + T-rich region. Gene content, order and orientation are identical to other reported tortricid mitogenomes. Analyses of nucleotide diversity, Ka/Ks, genetic distance and number of variable sites together suggest that nad6 is the fastest-evolving gene among the mitochondrial PCGs of Grapholita. Our analyses indicate that Grapholita, as presently defined, is not monophyletic, confirming previous morphological and multiple-gene studies, using mitogenomic evidence. Our study provides information on comparative mitogenomics of Tortricidae especially Grapholita.

线粒体基因组（有丝分裂基因组）已广泛用于系统发育和物种水平的进化研究。鳞翅目（Tortricidae）（卷蛾）包括Grapholita属在内，包含许多具有重要经济意义的物种，但对于大多数Grapholita物种而言，其有丝分裂基因组的研究仍很少。在这里，我们对Grapholita delineana（一种世界范围内的重要大麻害虫）的完整有丝分裂基因组进行测序和注释，并将其与可从GenBank获得的两个同类物种的有丝分裂基因组进行比较。该G. delineana有丝分裂基因组长15599 bp，包括37个典型的线粒体基因和一个富含A + T的区域。基因含量，顺序和方向与其他报道的类三叶虫有丝分裂基因组相同。核苷酸多样性，Ka / Ks，遗传距离和可变位点数目的分析共同表明，nad6是Grapholita线粒体PCG中发展最快的基因。我们的分析表明，根据线粒体基因组学证据，目前定义的Grapholita不是单系的，从而证实了先前的形态学和多基因研究。我们的研究提供了关于mi科（尤其是Grapholita）的比较线粒体学的信息。