Azadirachtin is a rapidly developing botanical pesticide with toxicity to arthropods. GST is a multifunctional biological enzyme that can remove peroxide and promote drug metabolism. In this study, RT-PCR technology was used to clone the GSTM3, GSTT1 and GSTD1 genes of Eriocheir hepuensis. The results showed that the coding sequence (CDS) lengths of the GSTM3, GSTT1 and GSTD1 genes were 675 bp, 687 bp and 666 bp, encoding 224, 228 and 221 amino acids, respectively. The deduced protein domain prediction showed that the GSTM3, GSTT1 and GSTD1 proteins of Eriocheir hepuensis had the GST-N-Mu and GST-C-family, GST-N-T and GST-C-T, and GST-N-D and GST-C-D domains, respectively. In normal tissues, the expression levels of GSTM3, GSTT1 and GSTD1 were highest in the hepatopancreas. Under azadirachtin stress, compared with the control group, the GSTM3, GSTT1 and GSTD1 genes showed a double peak (both at 6 h and 24 h) in the hepatopancreas and a single peak (peak of GSTD1 in gills at 9 h, others at 6 h) in the heart and gills, while the expression of GSTM3, GSTT1 and GSTD1 in the dead individuals in the experimental group was lower than that in the surviving individuals. These results showed that individual death under azadirachtin stress is related to its ability to regulate the expression of GSTM3, GSTT1 and GSTD1, and the three GST genes played an important role in Eriocheir hepuensis regulating azadirachtin stress.

印za素是一种快速发展的植物杀虫剂，对节肢动物有毒性。GST是一种多功能生物酶，可以去除过氧化物并促进药物代谢。在这项研究中，RT-PCR技术被用于克隆Eriocheir hepuensis的GSTM3，GSTT1和GSTD1基因。结果表明，GSTM3，GSTT1和GSTD1基因的编码序列（CDS）长度分别为675 bp，687 bp和666 bp，分别编码224、228和221个氨基酸。推导的蛋白质结构域的预测表明的GSTM3，GSTT1和GSTD1蛋白绒hepuensis分别具有GST-N-Mu和GST-C家族，GST-NT和GST-CT，以及GST-ND和GST-CD域。在正常组织中，GSTM3，GSTT1和GSTD1的表达水平在肝胰腺中最高。在印za素应力下，与对照组相比，GSTM3，GSTT1和GSTD1基因在肝胰腺中显示一个双峰（分别在6 h和24 h）和一个单峰（g中的GSTD1峰值）在9 h，其他在6 h）在心脏和腮中，而GSTM3，GSTT1和GSTD1的表达实验组中死者的存活率低于存活者。这些结果表明，印a素胁迫下的个体死亡与其调节GSTM3，GSTT1和GSTD1表达的能力有关，并且三个GST基因在调节中华绒ach素胁迫的中华绒螯蟹中起重要作用。