The impact of water temperature on the physiology of Channa punctata (Bloch, 1793) was evaluated in the present study. Fish were acclimated at 25 ± 1 °C and then exposed at six different temperatures: 10, 15, 20, 25, 30, and 35 °C. C. punctata exposed at 10, 15, and 20 °C showed 30, 21, and 11% reduced food consumption, respectively compared to 25 °C. Significantly higher respiratory burst and myeloperoxidase activities were found in fish exposed at 20 and 25 °C after 12 h of exposure compared to other treatments. Nitric oxide synthase was significantly higher at 25 °C after 12 h and at 25 and 30 °C exposed fish after 7 days compared to others. The reduced glutathione level was significantly higher at 25 °C compared to other treatments after 7 days of exposure. The thiobarbituric acid reactive substances level was minimum at 25 °C. Significantly lower antioxidant enzymes, catalase, glutathione peroxidase, and glutathione S-transferase were found in gills of fish exposed at 25 °C compared to others in both samples. The highest antioxidant enzyme levels were found at 10 °C. Heat shock protein (Hsp) 70 levels were significantly lower in liver and muscle of fish exposed at 25 °C compared to other treatments. The Hsp level was significantly higher at 35 and 30 °C exposed fish compared to others after 12 h, and the level reduced after 7 days in these treatments. Thermal stress affects food consumption rate, immune system, antioxidant enzymes, and enzyme systems in fish. The elevated Hsp70 level serves as a biomarker of stress in C. punctata.

在本研究中评估了水温对Channa punctata（Bloch，1793）生理的影响。鱼在25±1°C下适应环境，然后在六个不同的温度下暴露：10、15、20、25、30和35°C。刺与25°C相比，暴露于10、15和20°C的食物消耗分别减少了30、21和11％。与其他处理相比，暴露于20 h和25°C的鱼暴露12 h后，呼吸爆发和髓过氧化物酶活性显着提高。与其他相比，一氧化氮合酶在12 h后在25°C以及在7天后在25和30°C暴露的鱼中显着更高。暴露7天后，与其他治疗相比，在25°C下降低的谷胱甘肽水平明显更高。硫代巴比妥酸反应性物质的水平在25°C时最低。与两个样品中的其他样品相比，在25°C下暴露的鱼g中发现的抗氧化酶，过氧化氢酶，谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶低得多。在10°C时发现最高的抗氧化酶水平。与其他处理相比，在25°C下暴露的鱼的肝脏和肌肉中，热休克蛋白（Hsp）70的水平明显降低。在12小时后，暴露于35和30°C的鱼的Hsp水平明显高于其他鱼类，而在这些处理中7天后，Hsp水平降低。热应激会影响鱼类的食物消耗率，免疫系统，抗氧化酶和酶系统。Hsp70水平升高是应激的生物标志。C. punctata。