A dual-functional nanosysterm is developed by means of Chlorin e6 (Ce6) as photosensitizer and 1,3-Diphenylisobenzofuran (DPBF) as fluorescent singlet oxygen (¹O₂) probe. Under 660 nm laser irradiation, Ce6 exhibites efficient ¹O₂ generation, and subsequently the production of ¹O₂ is assessed by the ratiometric fluorescence of PFO and DPBF under one-photon and two-photon excitation mode. The nanoparticles with excellent biocompatibility can be internalized into Hela cells and applied for tumor treatment. For intracellular PDT, the nanoparticles perform a high phototoxicity, while the PDT proccess can be evaluated in time by monitoring fluorescence signals of DPBF. This theranostic nanosysterm provides a facile strategy to fabricate ¹O₂-detection PDT, which can realize accurate and efficient photodynamic therapy based on singlet oxygen detection.

^{以Chlorin e6 (Ce6)为光敏剂，1,3-二苯基异苯并呋喃(DPBF)为荧光单线态氧( 1} O ₂ )探针，开发了一种双功能纳米系统。在 660 nm 激光照射下，Ce6 表现出高效的¹ O ₂生成，随后产生¹ O ₂通过单光子和双光子激发模式下 PFO 和 DPBF 的比率荧光来评估。具有优异生物相容性的纳米颗粒可以内化到Hela细胞中并应用于肿瘤治疗。对于细胞内PDT，纳米颗粒具有较高的光毒性，而PDT过程可以通过监测DPBF的荧光信号及时评估。该治疗诊断纳米系统为制备¹ O ₂检测PDT提供了一种简便的策略，可以实现基于单线态氧检测的准确高效的光动力治疗。