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Structure of β-glucan from Tibetan hull-less barley and its in vitro fermentation by human gut microbiota
Chemical and Biological Technologies in Agriculture ( IF 5.2 ) Pub Date : 2021-02-17 , DOI: 10.1186/s40538-021-00212-z Chenxi Nie , Xin Yan , Xiaoqing Xie , Ziqi Zhang , Jiang Zhu , Yangfan Wang , Xinlei Wang , Nan Xu , Yiwen Luo , Zhen Sa , Xinyang Li , Huicui Liu , Baoming Tian , Qingyu Ma , Min Zhang , Zhifei Chen , Juxiu Li
Chemical and Biological Technologies in Agriculture ( IF 5.2 ) Pub Date : 2021-02-17 , DOI: 10.1186/s40538-021-00212-z Chenxi Nie , Xin Yan , Xiaoqing Xie , Ziqi Zhang , Jiang Zhu , Yangfan Wang , Xinlei Wang , Nan Xu , Yiwen Luo , Zhen Sa , Xinyang Li , Huicui Liu , Baoming Tian , Qingyu Ma , Min Zhang , Zhifei Chen , Juxiu Li
The influence of β-glucan on the human gut microbiota is closely related to the physicochemical structure of β-glucan. We purified a homogeneous water-soluble polysaccharide from Tibetan hull-less barley 25 and studied its structure and the in vitro fermentation profile. Analysis by gas chromatography (GC), Fourier-transformed infrared (FT-IR), nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), and atomic force micrograph (AFM) helped determine the hull-less barley polysaccharide to be a β-glucan (molecular weight: 3.45 × 104 Da), which was further characterized as mixed-linkage (1 → 3)(1 → 4)-linked β-d-glucans. SEM images demonstrated an intricate web structure of the hull-less barley polysaccharide, while the AFM images revealed the presence of small spherical particles in its structure. In addition, the microbiota composition of the hull-less barley polysaccharide group was found to be altered, wherein the abundance of Pantoea, Megamonas, Bifidobacterium, and Prevotella-9 were increased. On the other hand, in vitro fermentation revealed that hull-less barley polysaccharide significantly decreased the pH value and increased the production of acetate, propionate, and butyrate. Hull-less barley polysaccharide is a type of dietary fiber, and its analysis suggested that it may serve as a prebiotic food supplement for the regulation of the gut microbiota.
更新日期：2021-02-17
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