Nanoplastic sizes and numbers: quantification by single particle tracking,Environmental Science: Nano

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Nanoplastic sizes and numbers: quantification by single particle tracking
Environmental Science: Nano ( IF 5.8 ) Pub Date : 2021-2-17 , DOI: 10.1039/d0en00951b
Robert Molenaar _{1,

2,

3,

4,

5} , Swarupa Chatterjee _{1,

2,

3,

4,

5} , Bjorn Kamphuis _{1,

2,

3,

4,

5} , Ine M. J. Segers-Nolten _{1,

2,

3,

4,

5} , Mireille M. A. E. Claessens _{1,

2,

3,

4,

5} , Christian Blum _{1,

2,

3,

4,

5}

Affiliation

Plastic particles have been found almost everywhere in the environment, in oceans, terrestrial water bodies, sediments and air. The extent of this unwanted contamination is difficult to fully capture. Existing quantification methods focus on the detection of millimeter to micrometer sized plastic particles, while plastic breakdown processes continue to smaller, nanometer sized, particles. For these nanoplastics, methods that are inexpensive and can be (semi-) automated for high throughput analysis of dilute nanoplastic particle suspensions, are lacking. Here we combine sensitive fluorescence video microscopy, NileRed staining of plastic particles, and single particle tracking (SPT) to count and size nanoplastics. With this approach we show that particle diameters as low as 45 nm can be quantified, mixing ratios of differently sized particles can be recovered, and number concentrations as low as 2 × 10⁶ particles per ml can be determined. These results indicate that this approach is promising for quantifying the sizes and concentrations of nanoplastics released from consumer and medical plastics and potentially in environmental samples.

中文翻译：

纳米塑料的大小和数量：通过单颗粒跟踪进行定量

在环境，海洋，陆地水体，沉积物和空气中几乎所有地方都发现了塑料颗粒。这种有害污染的程度很难完全捕获。现有的量化方法侧重于检测毫米至微米大小的塑料颗粒，而塑料分解过程仍在继续对较小的纳米大小的颗粒进行检测。对于这些纳米塑料，缺乏廉价的方法，并且该方法可以（半）自动化进行稀纳米塑料颗粒悬浮液的高通量分析。在这里，我们结合了灵敏的荧光视频显微镜，塑料颗粒的NileRed染色和单颗粒跟踪（SPT）来计数和确定纳米塑料的大小。通过这种方法，我们证明了可以量化低至45 nm的粒径，可以确定每毫升^6个颗粒。这些结果表明，这种方法有望用于量化从消费塑料和医用塑料以及可能在环境样品中释放的纳米塑料的尺寸和浓度。

更新日期：2021-02-17

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