Development of immunochromatographic strips for the detection of dicofol,Analyst

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Development of immunochromatographic strips for the detection of dicofol
Analyst ( IF 3.6 ) Pub Date : 2021-1-25 , DOI: 10.1039/d0an02238a
Jie Liu _{1,

2,

3,

4,

5} , Shanshan Song _{1,

2,

3,

4,

5} , Aihong Wu _{1,

2,

3,

4,

5} , Hua Kuang _{1,

2,

3,

4,

5} , Liqiang Liu _{1,

2,

3,

4,

5} , Jing Xiao _{4,

6,

7,

8} , Chuanlai Xu _{1,

2,

3,

4,

5}

Affiliation

In this study, a monoclonal antibody (mAb) against dicofol was developed to prepare immunochromatographic strips (ICAs) for the detection of dicofol residues in fruit and vegetables. The mAb exhibited high affinity and high sensitivity, with an affinity constant of 2.96 × 10¹⁰ and a limit of detection of 3.142 ng mL⁻¹. A cross reactivity test revealed that the mAb also had good specificity for dicofol. This ICA method gave a visible limit of detection of 50 ng g⁻¹, and a cut-off value of 500 ng g⁻¹ for the detection of dicofol in both apple and cucumber with the naked eye. Importantly, the results here are consistent with results obtained using liquid chromatography mass spectrometry and ic-ELISAs indicating that this ICA method is reliable and practical when used for the detection of dicofol in fruit and vegetables.

中文翻译：

用于检测三氯杀螨醇的免疫色谱条的开发

在这项研究中，针对三氯杀螨醇的单克隆抗体（mAb）被开发来制备用于检测水果和蔬菜中三氯杀螨醇残留的免疫色谱条（ICAs）。mAb具有高亲和力和高灵敏度，亲和常数为2.96×10 ¹⁰，检出限为3.142 ng mL ^-1。交叉反应测试表明，mAb对三氯杀螨醇也具有良好的特异性。这种ICA方法的可见极限为50 ng g ^-1，截止值为500 ng g ^-1用肉眼检测苹果和黄瓜中的三氯杀螨醇。重要的是，这里的结果与使用液相色谱质谱法和ic-ELISAs获得的结果一致，表明该ICA方法用于检测水果和蔬菜中的三氯杀螨醇是可靠且实用的。

更新日期：2021-02-17

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