The fluorescence quantitative analysis method of a solution is widely applied in chemical analysis, clinical medicine testing, environmental monitoring, food safety detection, and so on. It is based on the linear relationship between the intensity of fluorescence emission and the concentration of the substance in solution. Without consideration of the spatial attenuation effect of excitation light, it is applied only to a dilute solution. In this research, a fluorescence emission model is established based on the interaction and propagation law between the excitation light and the fluorescent substances. The spatial attenuation effect of excitation light is analyzed by an element analysis method, and the spatial intensity distribution of fluorescence is revealed. Further, a high accuracy model between the received fluorescence intensity and concentration is obtained. Applications of this model and further design will allow for high throughput fluorescence analysis and the analysis of fluorescent substances with ultra-wide range concentration, such as on-line testing fluorescent dyes in the textile industry, monitoring protein plasma in biomedical field, and high-throughput DNA fluorescence analysis etc. As an example, based on this model, an ultra-wide concentration range (0.02 − 250 mg/L) detection of tryptophan with high accuracy (R² = 0.9994, RRMSE = 0.0356) is realized.

中文翻译：

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考虑激发光空间衰减效应的荧光发射空间强度分布模型

溶液的荧光定量分析方法广泛应用于化学分析、临床药物检测、环境监测、食品安全检测等。它基于荧光发射强度与溶液中物质浓度之间的线性关系。不考虑激发光的空间衰减效应，仅适用于稀溶液。本研究基于激发光与荧光物质之间的相互作用和传播规律，建立了荧光发射模型。通过元素分析法分析激发光的空间衰减效应，揭示荧光的空间强度分布。更远，获得了接收荧光强度和浓度之间的高精度模型。该模型的应用和进一步设计将允许高通量荧光分析和超宽浓度范围的荧光物质分析，如纺织行业的在线检测荧光染料，生物医学领域的蛋白质血浆监测，以及高浓度荧光物质的分析。通量DNA荧光分析等。 例如，基于该模型，超宽浓度范围（0.02 - 250 mg/L）色氨酸的高精度检测（R² = 0.9994，RRMSE = 0.0356) 实现。