Temporal lobe epilepsy (TLE) is the most familiar localized epilepsy in children. MicroRNAs (miRNAs) are essential for the inhibition or promotion of numerous diseases. This study aimed to detect the expression of miR-135b-5p and primarily uncover its underlying function and mechanism in children with TLE. Quantitative real-time polymerase chain reaction was used to evaluate the expression of miR-135b-5p in children with TLE and in a rat model of epilepsy. MTT assay and flow cytometric apoptosis assay were conducted to evaluate the effects of miR-135b-5p on cell viability and apoptosis. Additionally, the dual luciferase reporter assay was performed to confirm the direct target of miR-135b-5p. Our data showed that the expression of miR-135b-5p was significantly decreased in children with TLE and in the epileptic rat neuron model. The dysregulation of miR-135b-5p could serve as a promising diagnostic biomarker for children with TLE. The overexpression of miR-135b-5p moderated the adverse influence on cell viability and apoptosis induced by magnesium-free medium. SIRT1 was identified as a target gene of miR-135b-5p. These results proved that miR-135b-5p might serve as a potential diagnostic biomarker in children with TLE. Overexpression of miR-135b-5p alleviates the postepileptic influence on cell viability and apoptosis by targeting SIRT1.
Dev Neurosci

中文翻译：

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miR-135b-5p的临床意义及其在颞叶癫痫患儿海马神经元增殖和凋亡中的作用

颞叶癫痫 (TLE) 是儿童最熟悉的局部癫痫。MicroRNAs (miRNAs) 对于抑制或促进多种疾病是必不可少的。本研究旨在检测miR-135b-5p的表达，初步揭示其在儿童TLE中的潜在功能和机制。定量实时聚合酶链反应用于评估 miR-135b-5p 在 TLE 儿童和癫痫大鼠模型中的表达。进行MTT测定和流式细胞术凋亡测定以评估miR-135b-5p对细胞活力和凋亡的影响。此外，还进行了双荧光素酶报告基因检测以确认 miR-135b-5p 的直接靶标。我们的数据显示，TLE 儿童和癫痫大鼠神经元模型中 miR-135b-5p 的表达显着降低。miR-135b-5p 的失调可作为 TLE 儿童的有希望的诊断生物标志物。miR-135b-5p 的过表达减轻了对无镁培养基诱导的细胞活力和细胞凋亡的不利影响。SIRT1被确定为 miR-135b-5p 的靶基因。这些结果证明 miR-135b-5p 可能作为 TLE 儿童的潜在诊断生物标志物。miR-135b-5p 的过表达通过靶向SIRT1减轻了癫痫后对细胞活力和细胞凋亡的影响。
开发神经科学