Melanoma is considered to be one of the most aggressive human tumors. Thus, early molecular diagnosis with risk factor stratification could be an efficacious strategy to increase the survival rates in affected patients. Murine cell lines B16-F1, B16-4A5, and S91 clone M3 are the ones most commonly applied in melanoma research. However, genetic peculiarities of these 3 cell lines have not been studied in detail before. Here, we closed this gap by molecular cytogenetic and array-comparative genomic hybridization studies and the translation of the characterized imbalances into the human genome. This study revealed severely rearranged karyotypes with in parts similar imbalances for all 3 cell lines. Interestingly, they involve genes known to play major roles in human melanoma. These are specifically the oncogenes and tumor suppressor genes, being associated with aggressive forms of melanoma. B16-F1, B16-4A5, and S91 clone M3 revealed aberrations which were similarly observed in human eye and skin but not in human uveal melanoma. Thus, they can be considered as model systems for advanced eye and skin melanoma.
Cytogenet Genome Res

中文翻译：

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小鼠黑素瘤细胞系S91克隆M3和B16-F1及其变异B16-4A5的分子细胞遗传学表征

黑色素瘤被认为是最具侵略性的人类肿瘤之一。因此，采用危险因素分层进行早期分子诊断可能是提高患病患者生存率的有效策略。小鼠细胞系B16-F1，B16-4A5和S91克隆M3是黑色素瘤研究中最常用的细胞系。然而，这3种细胞系的遗传特性以前没有被详细研究过。在这里，我们通过分子细胞遗传学和与阵列比较的基因组杂交研究，以及将特征性失衡转化为人类基因组，填补了这一空白。这项研究揭示了严重重排的核型，在所有3种细胞系中部分失衡相似。有趣的是，它们涉及已知在人类黑色素瘤中起主要作用的基因。具体来说就是癌基因和抑癌基因，与侵袭性黑色素瘤有关。B16-F1，B16-4A5和S91克隆M3揭示了在人眼和皮肤中相似地观察到的畸变，但在人葡萄膜黑色素瘤中却没有观察到。因此，它们可以被认为是晚期眼睛和皮肤黑色素瘤的模型系统。
细胞遗传基因组研究