Transcriptome Analysis Reveals the Symbiotic Mechanism of Ustilago esculenta-Induced Gall Formation of Zizania latifolia,Molecular Plant-Microbe Interactions

当前位置： X-MOL 学术 › Mol. Plant Microbe Interact. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Transcriptome Analysis Reveals the Symbiotic Mechanism of Ustilago esculenta-Induced Gall Formation of Zizania latifolia
Molecular Plant-Microbe Interactions ( IF 3.2 ) Pub Date : 2021-01-05 , DOI: 10.1094/mpmi-05-20-0126-r
Jie Li _{1,

2} , Zhiyuan Lu _{1,

2} , Yang Yang _{1,

2} , Jinfeng Hou _{1,

2,

3} , Lingyun Yuan _{1,

2,

3} , Guohu Chen _{1,

2,

3} , Chenggang Wang _{1,

2,

3} , Shaoke Jia _{1,

2} , Xuming Feng _{1,

2} , Shidong Zhu _{1,

2,

3}

Affiliation

Zizania latifolia is a perennial aquatic vegetable, whose symbiosis with the fungus Ustilago esculenta (member of Basidiomycota, class Ustilaginaceae) results in the establishment of swollen gall formations. Here, we analyzed symbiotic relations of Z. latifolia and U. esculenta, using a triadimefon (TDF) treatment and transcriptome sequencing (RNA-seq). Specifically, accurately identify the whole growth cycle of Z. latifolia. Microstructure observations showed that the presence of U. esculenta could be clearly observed after gall formation but was absent after the TDF treatment. A total of 17,541 differentially expressed genes (DEGs) were identified, based on the transcriptome. According to gene ontology term and Kyoto Encyclopedia of Genes and Genomes pathway results, plant hormone signal transduction, and cell wall–loosening factors were all significantly enriched due to U. esculenta infecting Z. latifolia; relative expression levels of hormone-related genes were identified, of which downregulation of indole 3-acetic acid (IAA)-related DEGs was most pronounced in JB_D versus JB_B. The ultra–high performance liquid chromatography analysis revealed that IAA, zeatin+trans zeatin riboside, and gibberellin 3 were increased under U. esculenta infection. Based on our results, we proposed a hormone–cell wall loosening model to study the symbiotic mechanism of gall formation after U. esculenta infects Z. latifolia. Our study thus provides a new perspective for studying the physiological and molecular mechanisms of U. esculenta infection of Z. latifolia causing swollen gall formations as well as a theoretical basis for enhancing future yields of cultivated Z. latifolia.

The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. 2021

中文翻译：

转录组分析揭示了til草（Ustilago esculenta）诱导的Zizania latifolia胆汁形成的共生机制。

Zizania latifolia是多年生的水生蔬菜，其与真菌Ustilago esculenta（Basidiomycota的成员，Ustilaginaceae类）共生，导致形成了肿胀的胆汁。在这里，我们使用三唑酮（TDF）处理和转录组测序（RNA-seq）分析了Z. latifolia和U. esculenta的共生关系。具体来说，准确地确定Z. latifolia的整个生长周期。显微组织观察表明，存在着U. esculenta在形成胆汁后可以清楚地观察到，但在TDF处理后则没有。基于转录组，总共鉴定了17,541个差异表达基因（DEG）。根据基因本体学术语和《京都基因与基因组百科全书》途径的结果，由于U. esculenta感染了Z. latifolia，植物激素信号转导和细胞壁松动因子均显着丰富。确定了激素相关基因的相对表达水平，其中与吲哚3-乙酸（IAA）相关的DEG的下调在JB_D与JB_B中最明显。超高效液相色谱分析显示，IAA，玉米素+反式玉米素核苷，和赤霉素3被下增加U.星虫感染。根据我们的研究结果，我们提出了一种激素-细胞壁松弛模型，以研究U. esculenta感染Z. latifolia后胆汁形成的共生机制。因此我们的研究提供了一个新的视角，为研究导致胆囊形成肿胀的Z. latifolia的U. esculenta感染的生理和分子机制，以及为提高栽培Z. latifolia的未来产量提供理论依据。