Abstract

The antimicrobial activity of an experimental solution containing essential oil of Lippia sidoides for denture cleaning was evaluated by (1) minimum inhibitory (MIC) and fungicidal/bactericidal concentration (MFC/MBC) tests against Candida albicans, Staphylococcus aureus, and Pseudomona aeruginosa; (2) the metabolic activity of C. albicans biofilm formed on flat-bottom microplates and denture base specimens based on the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); and (3) scanning electron microscopy, to evaluate the fungal biofilm morphology. The solution showed antimicrobial action against the pathogens tested (C. albicans - MIC and MFC: 19.53 µg ml⁻¹, S. aureus - MIC and MBC: 78.12 µg ml⁻¹, P. aeruginosa - MIC: 625 µg ml⁻¹, MBC: 2,500 µg ml⁻¹), reduced the metabolic activity of C. albicans biofilm up to 97%, and caused cell wall damage at low concentrations (195.3–390.6 µg ml⁻¹) and in short time periods (20 min). Therefore, the experimental solution has the potential to be used as an alternative in the prevention and treatment of denture-induced infections.

摘要

通过（1）对白色念珠菌，金黄色葡萄球菌和铜绿假单胞菌的最小抑菌（MIC）和杀真菌/杀菌浓度（MFC / MBC）试验评估了含有利普亚ido子精油的实验溶液对义齿清洁的抗菌活性; （2）基于3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）的还原，在平底微孔板和义齿基体标本上形成的白色念珠菌生物膜的代谢活性；（3）扫描电子显微镜，以评估真菌生物膜的形态。该溶液对被测病原体（白色念珠菌）具有抗菌作用。- MIC和MFC：19.53微克毫升^-1，金黄色葡萄球菌- MIC和MBC：78.12微克毫升^-1，铜绿假单胞菌- MIC：625微克毫升^-1，MBC：2500微克毫升^-1），降低的代谢活性的白色念珠菌生物膜高达97％，并且在低浓度下引起细胞壁损伤（195.3-390.6微克毫升^-1），并在短的时间段（20分钟）。因此，该实验解决方案有潜力在预防和治疗义齿引起的感染中用作替代方案。