Polar and subpolar regions are known for their particular vulnerability and sensitivity to the detrimental effects of non-indigenous species, which is well exemplified by the Nootka lupin (Lupinus nootkatensis) spread in Iceland. Since understanding the population and ecological genetics of invasive alien species offers hope for counteracting harmful biological invasions, the objective of the present study was to investigate interspecific variation in L. nootkatensis in Iceland in relation to a native population in Alaska. Moreover, we aimed to assess whether internal transcribed spacer 2 (ITS2) has sufficient phylogenetic applicability for a large-scale screening of the genetic diversity of a non-indigenous population of this species. This study, which is the first attempt to investigate the genetic diversity of the Nootka lupin in Iceland, included plant samples from eight locations in Iceland and one in Alaska. The analyses included genotyping by sequencing of the 417-nucleotide fragment of the 5.8S ribosomal RNA, ITS2 and part of the large subunit ribosomal RNA (GenBank MT026578-MT026580, MT077004). The main findings showed the presence of five previously unexplained single-nucleotide polymorphisms (SNPs); however, their discriminatory power for Icelandic populations was relatively low, since polymorphism information content (PIC) values ranged from 0.0182 to 0.0526, with average heterozygosity 0.0296. Concomitantly, analysis of multilocus genotypes (MLG) revealed sufficient differences in MLGs variants and their frequency to form genotypic patterns unique for Alaskan and Icelandic populations, revealing an internal genetic structure of the studied group. The proposed SNP panel needs to be supplemented with other nuclear and organellar markers.

极地和亚极地地区以其对非土著物种的有害影响的特殊脆弱性和敏感性而闻名，这在冰岛散播的Nootka lupine（Lupinus nootkatensis）就是很好的例证。由于了解外来入侵物种的种群和生态遗传学为抵抗有害生物入侵提供了希望，因此本研究的目的是调查诺特卡特氏菌的种间变异  与阿拉斯加的原住民有关。此外，我们旨在评估内部转录间隔区2（ITS2）是否具有足够的系统发育适用性，可以大规模筛选该物种非土著种群的遗传多样性。这项研究是调查冰岛Nootka lupine遗传多样性的首次尝试，包括来自冰岛八个地点和阿拉斯加一个地点的植物样本。分析包括通过对5.8S核糖体RNA，ITS2和部分大亚基核糖体RNA（GenBank MT026578-MT026580，MT077004）的一部分的417个核苷酸片段进行测序进行基因分型。主要发现表明存在五个先前无法解释的单核苷酸多态性（SNP）。但是，它们对冰岛人的歧视能力相对较低，因为多态信息含量（PIC）值的范围从0.0182到0.0526，平均杂合度为0.0296。同时，对多基因座基因型（MLG）的分析显示，MLG变体及其频率形成了阿拉斯加和冰岛人种群独特的基因型模式，存在足够的差异，从而揭示了该研究组的内部遗传结构。拟议的SNP小组需要补充其他核和细胞标记物。