Meloidogyne incognita is the most frequently reported specie in the root-knot nematode (RKN) responsible for damage to several different crops worldwide. The interaction between M. incognita and host plants involves the secretions of molecular factors from the nematode, which mainly suppress the defense response and promote plant parasitism. On the other hand, several plant elements are associated with the immune defense system that opposes nematode infection. In this study, the interaction of the Mi-EFF1/Minc17998 effector with the soybean GmHub6 (Glyma.17G099100; TCP14) protein was identified and characterized in vivo and in planta. Data showed that the GmHub6 gene is upregulated by M. incognita infection in a nematode-resistant soybean genotype (PI595099) compared to a susceptible cultivar (BRS133). As a result, the Arabidopsis thaliana AtHub6 mutant (AtHub6^KO) line (AT3G47620, an orthologous gene of GmHub6) exhibited normal vegetative development of the plant but was more susceptible to M. incognita. Thus, since the soybean and A. thaliana Hub6 proteins are TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factors involved in plant development and morphogenesis modulation, flowering time regulation, and activation of the plant immune system, our data suggest that the interaction of Mi-EFF1/Minc17998 and Hub6 proteins is associated with an increase in plant susceptibility to nematode infection during parasitism. It is suggested that this interaction may prevent the nuclear localization or disturb the activity of GmHub6 as a typical transcription factor modulating the cell cycle of the plant, avoid the activation of the host's defense response, and successfully promote parasitism. Our findings indicate the potential of the Mi-EFF1/Minc17998 effector for the development of biotechnological tools based on the RNA interference approaches and GmHub6 gene overexpression for the RKN control.

根结线虫（RKN）中最常见的物种是根结线虫（Meloidogyne incognita），该物种负责破坏全球几种不同的农作物。之间的相互作用南方根结线虫和寄主植物涉及从线虫分子因素，主要抑制的防御反应和促进植物寄生的分泌物。另一方面，几种植物成分与抵抗线虫感染的免疫防御系统有关。在这项研究中，Mi-EFF1 / Minc17998效应子与大豆GmHub6（Glyma.17G099100; TCP14）蛋白的相互作用已在体内和植物体内得到鉴定和表征。数据显示，M。incognita上调了GmHub6基因。与易感品种（BRS133）相比，抗线虫大豆基因型（PI595099）感染。其结果是，在拟南芥AtHub6突变体（AtHub6 ^KO）线（AT3G47620，的直系同源基因GmHub6）所表现出的设备的正常营养发育但是更敏感南方根结线虫。因此，由于大豆和拟南芥Hub6蛋白是TEOSINTE BRANCHED / CYCLOIDEA / PCF（TCP）转录因子，参与植物发育和形态发生调节，开花时间调节以及植物免疫系统的激活，我们的数据表明Mi-EFF1 / Minc17998与Hub6蛋白的相互作用是与寄生期间植物对线虫感染的敏感性增加有关。提示这种相互作用可能阻止核定位或干扰GmHub6的活性，而后者是调节植物细胞周期的典型转录因子，避免了宿主防御反应的激活，并成功地促进了寄生。我们的发现表明，Mi-EFF1 / Minc17998效应子在开发基于RNA干扰方法和GmHub6的生物技术工具方面的潜力 RKN对照的基因过表达。