Biphasic calcium phosphate ceramics (BCPs) have been extensively used as a bone graft in dental clinics to reconstruct lost bone in the jaw and peri-implant hard tissue due to their good bone conduction and similar chemical structure to the teeth and bone. However, BCPs are not inherently osteoinductive and need additional modification and treatment to enhance their osteoinductivity. The present study aims to develop an innovative strategy to improve the osteoinductivity of BCPs using unique features of zeolitic imidazolate framework-8 (ZIF8). In this method, commercial BCPs (Osteon II) were pre-coated with a zeolitic imidazolate framework-8/polydopamine/polyethyleneimine (ZIF8/PDA/PEI) layer to form a uniform and compact thin film of ZIF8 on the surface of BCPs. The surface morphology and chemical structure of ZIF8 modified Osteon II (ZIF8-Osteon) were confirmed using various analytical techniques such as XRD, FTIR, SEM, and EDX. We evaluated the effect of ZIF8 coating on cell attachment, growth, and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADSCs). The results revealed that altering the surface chemistry and topography of Osteon II using ZIF8 can effectively promote cell attachment, proliferation, and bone regeneration in both in vitro and in vivo conditions. In conclusion, the method applied in this study is simple, low-cost, and time-efficient and can be used as a versatile approach for improving osteoinductivity and osteoconductivity of other types of alloplastic bone grafts.

双相磷酸钙陶瓷（BCP）由于其良好的骨传导性以及与牙齿和骨骼相似的化学结构，已在牙科诊所中广泛用作骨移植物，以重建颌骨和种植体周围硬组织中的骨丢失。但是，BCP本身不是骨诱导性的，需要进行其他修饰和处理以增强其骨诱导性。本研究旨在开发一种创新的策略，以利用沸石咪唑盐骨架8（ZIF8）的独特功能来提高BCP的骨诱导性。在该方法中，将商用的BCP（Osteon II）预先涂有沸石咪唑酸酯骨架8 /聚多巴胺/聚乙烯亚胺（ZIF8 / PDA / PEI）层，以在BCP的表面上形成均匀且致密的ZIF8薄膜。使用各种分析技术（例如XRD，FTIR，SEM和EDX）确认了ZIF8修饰的Osteon II（ZIF8-Osteon）的表面形态和化学结构。我们评估了ZIF8涂层对人脂肪来源的间充质干细胞（hADSCs）的细胞附着，生长和成骨分化的影响。结果表明，使用ZIF8改变Osteon II的表面化学和形貌可有效促进两种细胞的附着，增殖和骨再生体外和体内条件。总之，本研究中使用的方法简单，低成本且省时，可作为改善其他类型异体骨移植物的骨诱导性和骨传导性的通用方法。