This study describes the physicochemical properties of soybean asolectin (ASO) liposomes loaded with phycocyanin (Phy) extracted from Spirulina sp. LEB 18. The effects of Phy in the liposomesʾ properties were investigated by Fourier transform infrared spectroscopy (FTIR), ¹H and ³¹P nuclear magnetic resonance (NMR), zeta (ζ)-potential, dynamic light scattering (DLS) and ultraviolet-visible (UV–vis) techniques. Phy restricted the motion of ASO polar and interface groups and disrupted the package arrangement of the lipid hydrophobic regions, as a likely effect of dipolar and π interactions related to its amino acid residues and pyrrole portions. These interactions were correlated to antiradical/antioxidant Phy responses obtained by 2,2-diphenyl-1-picrylhidrazil (DPPH) assay, thiobarbituric acid reactive substances (TBARS) and ferric reducing antioxidant power (FRAP) methods, and discussed to bring new chemical perspectives about Phy-loaded liposomes-related nutraceutical applications in inflammatory and viral infection processes.

本研究描述了负载有从螺旋藻中提取的藻蓝蛋白 (Phy) 的大豆缩乳凝集素 (ASO) 脂质体的理化特性。LEB 18. 通过傅里叶变换红外光谱 (FTIR)、¹ H 和³¹研究了 Phy 对脂质体特性的影响P 核磁共振 (NMR)、zeta (ζ) 电位、动态光散射 (DLS) 和紫外-可见 (UV-vis) 技术。Phy 限制了 ASO 极性和界面基团的运动，并破坏了脂质疏水区域的包装排列，这可能是与其氨基酸残基和吡咯部分相关的偶极和 π 相互作用的影响。这些相互作用与通过 2,2-二苯基-1-苦基肼 (DPPH) 测定、硫代巴比妥酸反应物质 (TBARS) 和铁还原抗氧化能力 (FRAP) 方法获得的抗自由基/抗氧化 Phy 反应相关，并讨论带来新的化学视角关于 Phy 负载脂质体相关的营养食品在炎症和病毒感染过程中的应用。