The focus on urogenital mycoplasmas as the possible etiologic agents of urogenital infections and syndromes, has increased in the last decade. Of these, Mycoplasma genitalium is proven to be pathogenic and sexually transmitted. We compared five commercially available assays for the detection of these organisms in urogenital mycoplasma culture specimen remnants. Stored specimen remnants were tested on Aptima Mycoplasma genitalium, Allplex™ STI Essential and CGMT, ResitancePlus^®MG and Allplex™ MG & AziR Assays. All positive M. genitalium specimens and culture negative, nucleic acid positive Ureaplasmas were sent to the National Microbiology Laboratory for confirmation. The Aptima Mycoplasma genitalium assay detected 7 M. genitalium infections, the Allplex™ STI-EA and the Allplex™ CGMT detected 6 M. genitalium positives, and the Allplex™MG and AziR and SpeeDx ResistancePlus^® MG detected 5 M. genitalium positives, four with macrolide resistant genes. The Allplex™ STI Essential assay was 100% sensitive and specific for Mycoplasma hominis and Ureaplasma targets. As seen in other studies, the Aptima Mycoplasma genitalium assay was 100% sensitive and specific for the detection of M. genitalium. The multiplex assays had lower sensitivities for M. genitalium detection (Allplex™ STI Essential and CGMT sensitivity of 85.71%; Allplex™ MG & AziR and SpeeDx ResistancePlus^® MG sensitivity of 71.43%) with high specificities of 100%. Assays tested have high sensitivities and specificities for the detection of urogenital mycoplasmas especially M. genitalium macrolide resistance markers. All labs wanting to perform onsite detection of these organisms will find an assay to easily fit into their workflow.

在过去十年中，对泌尿生殖道支原体作为泌尿生殖道感染和综合征的可能病原体的关注有所增加。其中，生殖支原体被证明是致病性和性传播的。我们比较了五种市售的检测方法，用于检测泌尿生殖支原体培养标本残余物中的这些微生物。在 Aptima Mycoplasma genitalium、Allplex™ STI Essential 和 CGMT、ResitancePlus ^® MG 和 Allplex™ MG & AziR Assays上测试了储存的残留样本。所有阳性生殖器标本和培养阴性、核酸阳性的脲原体均送国家微生物实验室确认。Aptima生殖支原体化验检测到 7 M. genitalium感染，Allplex™ STI-EA 和 Allplex™ CGMT 检测到 6  M. genitalium阳性，Allplex™MG 和 AziR 和 SpeeDx ResistancePlus ^® MG 检测到 5  M. genitalium阳性，其中四个具有大环内酯抗性基因. Allplex™ STI Essential 检测对人支原体和脲原体靶标具有 100% 的灵敏度和特异性。正如在其他研究中看到的那样，APTIMA生殖支原体测定为100％的敏感性和特异性用于检测生殖支原体。多重检测对生殖器支原体的敏感性较低检测（Allplex™STI本质和CGMT的85.71％的敏感性; Allplex™MG＆AziR和SpeeDx ResistancePlus ^® MG的71.43％的敏感性），用100％的高特异性。测试测定具有高灵敏度和特异性用于检测泌尿生殖支原体特别的生殖道支原体大环内酯类抗性标记。所有想要对这些生物进行现场检测的实验室都会找到一种可以轻松融入其工作流程的检测方法。