A novel fluorescence probe NA-LCX was rationally designed and synthesized for the sequential recognition of Cu²⁺ and H₂S by the combination of hydroxyl-naphthalene and diformylphenol groups. The response properties of NA-LCX for Cu²⁺ ions and H₂S with “on-off-on” manner were investigated by fluorescence emission spectra. A highly selective and sensitive response of complex NA-LCX-Cu²⁺ for H₂S over other competing amino acids was observed with a limit of detection at 2.79 μM. The stoichiometry of NA-LCX toward Cu²⁺ ions was determined to be 1 : 1 by the UV-Vis absorption spectrum, and the coordination configuration was calculated by density functional theory (DFT) calculations. Moreover, probe NA-LCX was applied successfully for the recognition of Cu²⁺ ions and H₂S in living cells.

中文翻译：

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基于铜配合物的选择性检测硫化氢荧光探针的设计与合成

一种新的荧光探针NA-LCX被合理地设计和用于顺序识别的Cu合成²⁺和H ₂被羟基萘和二甲酰基苯酚基团的组合S上。通过荧光发射光谱研究了NA-LCX对Cu ²⁺离子和H ₂ S“开-关-开”方式的响应特性。复杂的NA-LCX-Cu构成的高选择性和灵敏的响应²⁺用于h ₂与检测限观察到S以上其他竞争氨基酸在2.79  μ M.朝向铜NA-LCX的化学计量²⁺通过UV-Vis吸收光谱确定离子为1：1，并且通过密度泛函理论（DFT）计算来计算配位构型。此外，探针NA-LCX是为识别的Cu成功地应用于²⁺离子和H ₂在活细胞中S上。