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RNAScope in situ Hybridization as a Novel Technique for the Assessment of c-KIT mRNA Expression in Canine Mast Cell Tumor
Frontiers in Veterinary Science ( IF 2.6 ) Pub Date : 2021-01-04 , DOI: 10.3389/fvets.2021.591961
Davide De Biase 1 , Francesco Prisco 1 , Giuseppe Piegari 1 , Arianna Ilsami 1 , Ilaria d'Aquino 1 , Valeria Baldassarre 1 , Federica Zito Marino 2 , Renato Franco 2 , Serenella Papparella 1 , Orlando Paciello 1
Affiliation  

RNA is considered as an indicator of the dynamic genetic expression changes in a cell. RNAScope is a commercially available in situ hybridization assay for the detection of RNA in formalin-fixed paraffin-embedded tissue. In this work, we describe the use of RNAScope as a sensitive and specific method for the evaluation of c-KIT messenger RNA (mRNA) in canine mast cell tumor. We investigated the expression of c-KIT mRNA with RNAscope in 60 canine mast cell tumors (MCTs), comparing it with the histological grade and KIT immunohistochemical expression patterns. Our results showed an overall good expression of c-KIT mRNA in neoplastic cells if compared with control probes. We also observed a statistically significant correlation between histological grade and c-KIT mRNA expression. No correlations were found between KIT protein immunohistochemical distribution pattern and c-KIT mRNA expression or histological grade. Our results provide a reference basis to better understand c-KIT mRNA expression in canine MCTs and strongly encourage further studies that may provide useful information about its potential and significant role as a prognostic and predictive biological marker for canine MCTs clinical outcome.



中文翻译:


RNAScope 原位杂交作为评估犬肥大细胞肿瘤中 c-KIT mRNA 表达的新技术



RNA被认为是细胞内动态基因表达变化的指标。 RNAScope 是一种市售的原位杂交测定法,用于检测福尔马林固定石蜡包埋组织中的 RNA。在这项工作中,我们描述了使用 RNAScope 作为一种敏感且特异的方法来评估犬肥大细胞肿瘤中的 c-KIT 信使 RNA (mRNA)。我们使用 RNAscope 研究了 60 个犬肥大细胞肿瘤 (MCT) 中 c-KIT mRNA 的表达,并将其与组织学分级和 KIT 免疫组织化学表达模式进行比较。我们的结果表明,与对照探针相比,肿瘤细胞中 c-KIT mRNA 的总体表达良好。我们还观察到组织学分级和 c-KIT mRNA 表达之间存在统计学上显着的相关性。 KIT 蛋白免疫组织化学分布模式与 c-KIT mRNA 表达或组织学分级之间未发现相关性。我们的结果为更好地了解犬 MCT 中的 c-KIT mRNA 表达提供了参考基础,并强烈鼓励进一步的研究,这些研究可能提供有关其作为犬 MCT 临床结果的预后和预测生物标志物的潜在和重要作用的有用信息。

更新日期:2021-02-16
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