RNA is considered as an indicator of the dynamic genetic expression changes in a cell. RNAScope is a commercially available in situ hybridization assay for the detection of RNA in formalin-fixed paraffin-embedded tissue. In this work, we describe the use of RNAScope as a sensitive and specific method for the evaluation of c-KIT messenger RNA (mRNA) in canine mast cell tumor. We investigated the expression of c-KIT mRNA with RNAscope in 60 canine mast cell tumors (MCTs), comparing it with the histological grade and KIT immunohistochemical expression patterns. Our results showed an overall good expression of c-KIT mRNA in neoplastic cells if compared with control probes. We also observed a statistically significant correlation between histological grade and c-KIT mRNA expression. No correlations were found between KIT protein immunohistochemical distribution pattern and c-KIT mRNA expression or histological grade. Our results provide a reference basis to better understand c-KIT mRNA expression in canine MCTs and strongly encourage further studies that may provide useful information about its potential and significant role as a prognostic and predictive biological marker for canine MCTs clinical outcome.

RNA被认为是细胞内动态基因表达变化的指标。 RNAScope 是一种市售的原位杂交测定法，用于检测福尔马林固定石蜡包埋组织中的 RNA。在这项工作中，我们描述了使用 RNAScope 作为一种敏感且特异的方法来评估犬肥大细胞肿瘤中的 c-KIT 信使 RNA (mRNA)。我们使用 RNAscope 研究了 60 个犬肥大细胞肿瘤 (MCT) 中 c-KIT mRNA 的表达，并将其与组织学分级和 KIT 免疫组织化学表达模式进行比较。我们的结果表明，与对照探针相比，肿瘤细胞中 c-KIT mRNA 的总体表达良好。我们还观察到组织学分级和 c-KIT mRNA 表达之间存在统计学上显着的相关性。 KIT 蛋白免疫组织化学分布模式与 c-KIT mRNA 表达或组织学分级之间未发现相关性。我们的结果为更好地了解犬 MCT 中的 c-KIT mRNA 表达提供了参考基础，并强烈鼓励进一步的研究，这些研究可能提供有关其作为犬 MCT 临床结果的预后和预测生物标志物的潜在和重要作用的有用信息。