Abstract

The search for novel reaction media with environmental friendly properties is an area of great interest in enzyme catalysis. Water is the medium of biocatalysed processes, but due to its properties, sometimes the presence of organic (co)solvents is required. Cyrene^TM represents one of the newest approaches to this medium engineering. This polar solvent has been employed for the first time in biocatalysed reductions employing purified alcohol dehydrogenases. A set of α-ketoesters has been reduced to the corresponding chiral α-hydroxyesters with high conversions and optical purities, being possible to obtain good results at Cyrene contents of 30% v/v and working at substrate concentrations of 1.0 M in presence of 2.5% v/v of this solvent. At this concentration, the presence of Cyrene has a beneficial effect in the bioreduction conversion.

摘要

寻找具有环境友好特性的新型反应介质是酶催化领域非常感兴趣的领域。水是生物催化过程的介质，但由于其特性，有时需要存在有机（共）溶剂。Cyrene ^TM代表了这种中等工程的最新方法之一。这种极性溶剂首次用于使用纯化的醇脱氢酶进行生物催化还原。一组 α-酮酯被还原为相应的手性 α-羟基酯，具有高转化率和光学纯度，在 30% v/v的 Cyrene 含量下和在 2.5 的底物浓度为 1.0 M 时可以获得良好的结果% v/v这种溶剂。在该浓度下，Cyrene 的存在对生物还原转化具有有益作用。