Abstract

The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.05 mg ml⁻¹ CaneCPI-5, 0.1 mg ml⁻¹ CaneCPI-5 or 0.5 mg ml⁻¹ CaneCPI-5. They were incubated with inoculum (human saliva + McBain's saliva) for the first 8 h. From then until the end of the experiment, the enamel was exposed to McBain saliva with sucrose and, once a day, for 5 days, they were treated with the solutions. At the end of the experimental period, resazurin and viable plate count assays were performed. Enamel demineralization was also measured. All concentrations of CaneCPI-5 and CHX significantly reduced the activity of biofilms compared with PBS. For viable plate counts, all treatments similarly reduced the lactobacilli and total streptococci; for the mutans streptococci, 0.05 mg ml⁻¹ CaneCPI-5 performed better than CHX. All CaneCPI-5 concentrations significantly reduced the integrated mineral loss. This study represents the first step regarding the use of CaneCPI-5 within the concept of acquired enamel pellicle and biofilm engineering to prevent dental caries.

摘要

评估了CaneCPI-5的抗菌和防龋效果。用磷酸盐缓冲盐溶液（PBS），0.12％洗必太（CHX），0.05 mg ml ^-1 CaneCPI-5、0.1 mg ml ^-1 CaneCPI-5或0.5 mg ml ^-1处理90个牛牙釉质样品60 sCaneCPI-5。在最初的8小时内，将它们与接种物（人唾液+ McBain唾液）一起孵育。从那时起直到实验结束，将牙釉质暴露于含蔗糖的McBain唾液中，每天一次，持续5天，然后用溶液对其进行处理。在实验期结束时，进行刃天青和活板计数测定。还测量了牙釉质的脱矿质。与PBS相比，所有浓度的CaneCPI-5和CHX都显着降低了生物膜的活性。对于活板计数，所有处理均类似地减少了乳酸菌和总链球菌。对于变形链球菌，0.05 mg ml ^-1CaneCPI-5的表现优于CHX。所有的CaneCPI-5浓度都显着降低了综合矿物质流失。这项研究代表了在获得性牙釉质防护膜和生物膜工程学概念中使用CaneCPI-5预防龋齿的第一步。