Three endogenous cellulases from termite, Reticulitermes speratus KMT001,Archives of Insect Biochemistry and Physiology

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Three endogenous cellulases from termite, Reticulitermes speratus KMT001
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-15 , DOI: 10.1002/arch.21766
Hee‐Hoon Ahn ₁ , Tae‐Jong Kim ₁

Affiliation

Among termites, lower termites need symbiotic microorganisms in the digestive tract for digestion and cellulose metabolism. In this symbiotic relationship, the decomposition of cellulose is initiated by endoglucanase in termite salivary glands and completed by β‐glycosidase of symbiotic microorganisms in the hindgut. The expression of β‐glycosidase in lower termites has been reported in recent studies. The expression of two endoglucanases and one β‐glycosidase gene related to cellulose degradation was identified in Reticulitermes speratus, a lower termite, through transcriptomic analysis. The proposed enzyme activities of three identified cellulose degradation genes were confirmed by heterologous expression in Escherichia coli. In addition to the endoglucanase expressed in the salivary gland, additional endoglucanase and β‐glycosidase genes suggest that R. speratus performs the overall cellulose digestion using its own enzymes at all stages.

中文翻译：

来自白蚁的三种内源纤维素酶，Reticulitermes speratus KMT001

在白蚁中，下白蚁在消化道中需要共生微生物以进行消化和纤维素代谢。在这种共生关系中，纤维素的分解由白蚁唾液腺中的内切葡聚糖酶引发，并由后肠中的共生微生物的β-糖苷酶完成。最近的研究报道了低级白蚁中β-糖苷酶的表达。2个内切葡聚糖酶和β一个糖苷酶基因相关的纤维素降解的表达在鉴定黄胸散白蚁，低级白蚁，通过转录分析。通过在大肠杆菌中的异源表达，证实了三个已鉴定的纤维素降解基因的拟议酶活性。除了在唾液腺中表达的内切葡聚糖酶外，其他内切葡聚糖酶和β-糖苷酶基因还表明，R。speratus在所有阶段都利用其自身的酶来进行整个纤维素的消化。

更新日期：2021-03-11

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