Baltic mussels (Mytilus spp.) were exposed to the explosive trinitrotoluene (TNT) for 96 h (0.31-10.0 mg/L) and 21 d (0.31-2.5 mg/L). Bioaccumulation of TNT and its degradation products (2- and 4-ADNT) as well as biological effects ranging from the gene and cellular levels to behaviour were investigated. Although no mortality occurred in the concentration range tested, uptake and metabolism of TNT and responses in antioxidant enzymes and histochemical biomarkers were observed already at the lowest concentrations. The characteristic shell closure behaviour of bivalves at trigger concentrations led to complex exposure patterns and non-linear responses to the exposure concentrations. Conclusively, exposure to TNT exerts biomarker reponses in mussels already at 0.31 mg/L while effects are recorded also after a prolonged exposure although no mortality occurs. Finally, more attention should be paid on shell closure of bivalves in exposure studies since it plays a marked role in definining toxicity threshold levels.

波罗的海贻贝（Mytilusspp。）暴露于爆炸性三硝基甲苯（TNT）中96 h（0.31-10.0 mg / L）和21 d（0.31-2.5 mg / L）。研究了TNT及其降解产物（2-和4-ADNT）的生物蓄积以及从基因，细胞水平到行为的生物学效应。尽管在所测试的浓度范围内没有发生死亡，但是在最低浓度下已经观察到TNT的吸收和代谢以及抗氧化酶和组织化学生物标记物的反应。双壳类动物在触发浓度下的典型壳闭合行为导致复杂的暴露模式和对暴露浓度的非线性响应。最终，暴露于TNT对贻贝已经产生了0.31 mg / L的生物标记反应，而长时间暴露后也记录了作用，尽管没有发生死亡。最后，