Plant terpenes are the main functional agents in forest bathing and have great value in several industries. To uncover the seasonal variation of Cinnamomum camphora monoterpene emission and the difference among the chemotypes for promoting the development of forest bathing and exploitation of monoterpenes using the species, the emission of monoterpenes and related gene expression in the camphor chemotype, eucalyptol chemotype, linalool chemotype and borneol chemotype of C. camphora were analyzed in May (spring), July (summer) and November (autumn), and the stored monoterpenes and storage structure were investigated. Eleocytes were the storage structure of monoterpenes in C. camphora, which widely existed in the leaf palisade tissue and spongy tissue, as well as around the glands and vascular bundles. The 4 chemotypes of C. camphora released different monoterpene components which mainly derived from the immediate emission after synthesis. Camphor, eucalyptol, linalool and endo-borneol showed the uppermost emission rate and were the typical monoterpenes in the corresponding chemotype, respectively, due to the high expression of the genes encoding bornyl diphosphate synthase (E5.5.1.8), linalool synthase (TSP14) and (+)-neomenthol dehydrogenase (E1.1.1.208) for the biosynthesis of camphor, linalool and endo-borneol, respectively, and only expression of the gene encoding 1,8-cineole synthase (TSP-cin) for eucalyptol biosynthesis. Among the 4 chemotypes, the linalool chemotype showed the highest emission rate of monoterpenes in the same month (without significant difference with the eucalyptol chemotype in November), due to the high expression of the genes (dxs, dxr, ispF, gcpE, and GGPS) in methylerythritol-4-phosphate (MEP) pathway, α-terpineol synthase gene (E4.2.3.111) and TSP14. Among the 3 months, temperature was the remarkably varied meteorological condition. The highest monoterpene emission rate from the 4 chemotypes of C. camphora was detected in July, which should result from the up-regulated expression of related genes in monoterpene biosynthesis and improved vaporization and diffusion of stored monoterpenes under high temperature, with the former providing a major contribution.

植物萜烯是森林沐浴中的主要功能剂，在许多行业中都具有很大的价值。揭示樟树樟脑单萜排放的季节变化以及利用该物种促进森林沐浴和开发单萜的化学型之间的差异，樟脑化学型，桉树油化学型，芳樟醇化学型和樟脑化学型的单萜排放和相关基因表达在5月（春季），7月（夏季）和11月（秋季）对樟脑樟脑的冰片化学类型进行了分析，并研究了其储存的单萜和储存结构。内皮细胞是樟脑中单萜的储存结构广泛存在于叶栅栏组织和海绵状组织以及腺体和血管束周围。樟脑樟的4种化学型释放出不同的单萜成分，这些成分主要来自合成后立即释放。由于编码降冰片基二磷酸合酶（E5.5.1.8），芳樟醇合酶（TSP14）的基因高表达，樟脑，桉树油，芳樟醇和内降冰片显示最高的发射率，分别是相应化学型中的典型单萜。）和（+）-新薄荷醇脱氢酶（E1.1.1.208）分别用于樟脑，芳樟醇和内降冰片的生物合成，并且仅表达编码1,8-桉树脑合酶的基因（TSP-cin）用于桉树素的生物合成。在这4种化学型中，由于基因（dxs，dxr，ispF，gcpE和GGPS）的高表达，芳樟醇化学型在同一月份显示出最高的单萜类排放（与11月的桉树化学型无显着差异）。）（4-磷酸甲基赤藓糖醇（MEP）途径），α-松油醇合酶基因（E4.2.3.111）和TSP14。在三个月中，温度是明显变化的气象条件。樟脑的4种化学型的最高单萜排放速率 在7月份检测到这种细菌，这可能是由于单萜生物合成中相关基因的表达上调以及高温下储存的单萜的汽化和扩散改善所致，而前者则起了主要作用。