Significance: Hemoglobin oxygen saturation and red blood cell (RBC) tissue fraction are important parameters when assessing microvascular status. Functional information can be attained using temporally resolved measurements performed during stimulus–response protocols. Pointwise assessments can currently be conducted with probe-based systems. However, snapshot multispectral imaging (MSI) can be used for spatial–temporal measurements. Aim: To validate if hemoglobin oxygen saturation and RBC tissue fraction can be quantified using a snapshot MSI system and an inverse Monte Carlo algorithm. Approach: Skin tissue measurements from the MSI system were compared to those from a validated probe-based system during arterial and venous occlusion provocation on 24 subjects in the wavelength interval 450 to 650 nm, to evaluate a wide range of hemoglobin oxygen saturation and RBC tissue fraction levels. Results: Arterial occlusion results show a mean linear regression R2 = 0.958 for hemoglobin oxygen saturation. Comparing relative RBC tissue fraction during venous occlusion results in R2 = 0.925. The MSI system shows larger dynamic changes than the reference system, which might be explained by a deeper sampling including more capacitance vessels. Conclusions: The snapshot MSI system estimates hemoglobin oxygen saturation and RBC tissue fraction in skin microcirculation showing a high correlation (R2 > 0.9 in most subjects) with those measured by the reference method.

中文翻译：

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使用多光谱快照成像系统估计皮肤微循环血红蛋白氧饱和度和红细胞组织分数：验证研究

意义：血红蛋白氧饱和度和红细胞 (RBC) 组织分数是评估微血管状态时的重要参数。可以使用在刺激-响应协议期间执行的时间分辨测量来获得功能信息。目前可以使用基于探针的系统进行逐点评估。然而，快照多光谱成像 (MSI) 可用于时空测量。目的：验证是否可以使用快照 MSI 系统和逆蒙特卡罗算法量化血红蛋白氧饱和度和 RBC 组织分数。方法：在 450 至 650 nm 波长间隔内对 24 名受试者进行动脉和静脉阻塞激发期间，将来自 MSI 系统的皮肤组织测量值与来自经验证的基于探针的系统的测量值进行比较，评估各种血红蛋白氧饱和度和红细胞组织分数水平。结果：动脉闭塞结果显示血红蛋白氧饱和度的平均线性回归 R2 = 0.958。比较静脉闭塞期间的相对 RBC 组织分数，R2 = 0.925。MSI 系统显示出比参考系统更大的动态变化，这可能是通过更深的采样（包括更多电容容器）来解释的。结论：快照 MSI 系统估计皮肤微循环中的血红蛋白氧饱和度和 RBC 组织分数，显示出与参考方法测量的高度相关（大多数受试者的 R2 > 0.9）。比较静脉闭塞期间的相对 RBC 组织分数，R2 = 0.925。MSI 系统显示出比参考系统更大的动态变化，这可能是通过更深的采样（包括更多电容容器）来解释的。结论：快照 MSI 系统估计皮肤微循环中的血红蛋白氧饱和度和 RBC 组织分数，显示出与参考方法测量的高度相关（大多数受试者的 R2 > 0.9）。比较静脉闭塞期间的相对 RBC 组织分数，R2 = 0.925。MSI 系统显示出比参考系统更大的动态变化，这可能是通过更深的采样（包括更多电容容器）来解释的。结论：快照 MSI 系统估计皮肤微循环中的血红蛋白氧饱和度和 RBC 组织分数，显示出与参考方法测量的高度相关（大多数受试者的 R2 > 0.9）。