This study confirmed the association with inflammation-related proteins, mediators, and cytokines using saucerneol D from Saururus chinensis leaf, a useful ingredient increased through elicitor treatment. To confirm the anti-inflammatory effect, saucerneol D were treated with lipopolysaccharide, which induces pro-inflammatory factors in Raw 264.7 cell. The pro-inflammatory influences were measured by dint of chemical assay and western blotting as well as ELISA. As a result, the content of saucerneol D was changed when eicitor was treated by various concentration (1.5, and 3 mg/mL) in S. chinensis leaves. In addition, the expression levels of hyaluronidase and pro-inflammatory-related factors [nitric oxide (NO), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2)] were regulated according to the saucerneol D content in the elicitor-treated and non-treated groups. Therefore, after confirming that saucerneol D has an inhibitory effect on pro-inflammatory-related factors, saucerneol D was adjusted by concentration and compared with the control substance to verify the efficacy. Saucerneol D was adjusted to a concentration that did not toxic to macrophages through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Saucerneol D controlled at various concentrations inhibited iNOS and COX-2 proteins. NO produced by iNOS activity, prostaglandin E2 (PGE2), an inflammatory mediator produced by COX-2 activity, and pro-inflammatory cytokines [interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α)] were significantly suppressed. Therefore, it was confirmed that saucerneol D, an active ingredient increased by the elicitor treatment, could be used as a functional material that controls inflammatory factors.

中文翻译：

---

激发子处理的中华三色龙的香豆酚D对LPS刺激的Raw 264.7细胞促炎反应的炎症信号通路的响应

这项研究证实了使用Saururus chinensis叶片中的sathereneol D与炎症相关的蛋白质，介体和细胞因子的相关性。为了证实抗炎作用，用脂多糖处理了茶多酚D，其在Raw 264.7细胞中诱导促炎因子。通过化学测定和蛋白质印迹以及ELISA来测定促炎作用。结果，当以不同浓度（1.5和3mg / mL）处理中华S叶片中的起泡剂时，香豆酚D的含量改变。此外，透明质酸酶和促炎相关因子[一氧化氮（NO），诱导型一氧化氮合酶（iNOS），环氧基加氧酶和环加氧酶-2（COX-2）]根据引发剂处理组和未处理组中香豆酚D的含量进行调节。因此，在确认茶多酚D对促炎相关因子具有抑制作用之后，通过浓度调节茶多酚D，并与对照物质进行比较以验证功效。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定，将草油D调节至对巨噬细胞无毒的浓度。以不同浓度控制的香叶​​素D抑制iNOS和COX-2蛋白。iNOS活性产生的NO，前列腺素E2（PGE2），COX-2活性产生的炎性介质和促炎细胞因子[白介素-1β（IL-1β），IL-6，肿瘤坏死因子-α（TNF-α ）]被显着抑制。所以，